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- Table of Contents
HDAC5 antibody (A01230-6) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Flow Cytometry analysis of THP-1 cells using anti-HDAC5 antibody (A01230-6).
Overlay histogram showing THP-1 cells stained with A01230-6 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HDAC5 Antibody (A01230-6, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing THP-1 cells stained with A01230-6 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HDAC5 Antibody (A01230-6, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-HDAC5 Antibody Picoband®
HDAC5 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
HDAC5 antibody was positively detected by WB in:
human K562 whole cell, human PC-3 whole cell, rat liver tissue, mouse liver tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
HDAC5 antibody was positively detected by IHC in:
mouse small intestine tissue, human placenta tissue, human esophageal squamous carcinoma tissue, human lung cancer tissue, human rectal cancer tissue, human breast cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
HDAC5 antibody was positively detected by ICC/IF in:
A431 cell, U20S cell
HDAC5 antibody was positively detected by FC in:
THP-1 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human, Mouse
Immunocytochemistry/Immunofluorescence
5μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-HDAC5 Antibody Picoband®
View all HDAC5 Antibodies
View all HDAC5 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human HDAC5 recombinant protein (Position: R13-H1107).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
150 kDa
Calculated Molecular Weight
122.0 kDa
Background of HDAC5
Histone deacetylase 5, also called HDAC5 or KIAA0600, is an enzyme that in humans is encodes by the HDAC5 gene. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. This gene is mapped to 17q21.31. Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is though to be associated with colon cancer.
Cite This Product
Anti-HDAC5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01230-6)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Cow
96%
High Homology
Sheep
96%
High Homology
Dog
96%
High Homology
Rabbit
96%
High Homology
Mouse
95%
High Homology
Rat
95%
High Homology
Zebrafish
73%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for HDAC5 antibody
Immunohistochemistry (IHC) protocol for HDAC5 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for HDAC5 antibody
Flow Cytometry (FC) protocol for HDAC5 antibody
Publications
Application:
Species:
Upregulation of HDAC9 in hippocampal neurons mediates depression-like behaviours by inhibiting ANXA2 degradation
Published: 2023-09-10
Journal: CELLULAR AND MOLECULAR LIFE SCIENCES
Application: WB
Impact Factor: 8.00
Species: Mouse
PMID: 37690046
Review
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1 Review For Anti-HDAC5 Antibody Picoband®
IHC with HDAC5 Antibody Picoband® (A01230-6) showed clear staining and low background, with significantly higher HDAC5 levels in the cortex of Alzheimer’s mice, suggesting potential as a biomarker.
| SKU | A01230-6 |
|---|---|
| Application | Immunohistochemistry |
| Sample | Normal mouse brain and Alzheimer’s model mouse brain |
| Sample Processing Description | Paraffin-embedded normal mouse brain and Alzheimer’s model mouse brain. |
| Other Reagents | Goat serum, DAB chromogen |
| Primary Antibody | Anti-HDAC5 Antibody Picoband® |
| Primary Incubation | 1:400, overnight at 4 ℃ |
| Secondary Antibody | Two-step IHC kit |
| Secondary Incubation | 30 minutes in 37 ℃ |
| Detection | Imaging system: Leica DM2500 |
| Results Summary | The results showed that HDAC5 levels in the cortex were significantly higher in Alzheimer’s mice than in normal mice, suggesting its potential as a biomarker for further validation. |
Song Qian, Zhejiang Police College
Verified customer
Submitted 2026-01-26
Product Promise
We promise Anti-HDAC5 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01230-6
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 5, 2026