Anti-Histone deacetylase 8 HDAC8 Antibody

Product Name

Anti-Histone deacetylase 8 HDAC8 Antibody

View all Histone Deacetylase 8/HDAC8 Antibodies

SKU/Catalog Number

PA1591

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Histone deacetylase 8 HDAC8 Antibody catalog # PA1591. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Histone deacetylase 8 HDAC8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1591)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human HDAC8, different from the related mouse and rat sequences by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1591 is reactive to HDAC8 in Human, Mouse, Rat

Applications

PA1591 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

41.758kDa

Background of Histone Deacetylase 8/HDAC8

HDAC8, Histone deacetylase 8, is an enzyme that in humans is encoded by the HDAC8 gene, and is biologically involved in skull morphogenesis and metabolic control of the ERR-alpha/PGC1-alpha transcriptional complex. Sequence analysis predicted that the 377-amino acid HDAC8 protein contains the 9 conserved HDAC blocks that are presumably important for catalytic function. HDAC8 shares 54% sequence similarity with HDAC1 and HDAC2 and 39% similarity with HDAC3, making it a class I HDAC. Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Click image to see more details

Anti-HDAC8 antibody, PA1591, Western blotting
All lanes: Anti HDAC8 (PA1591) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle: Tissue Lysate at 50ug
Lane 2: A549 Whole Cell Lysate at 40ug
Predicted bind size: 42KD
Observed bind size: 42KD

Click image to see more details

Anti-HDAC8 antibody, PA1591, IHC(P)
IHC(P): Human Lung Cancer Tissue

Gene/Protein Information For HDAC8 (Source: Uniprot.org, NCBI)

Alternative Names

EC 3.5.1.98; HD8; HDAC8; HDACL1; Histone Deacetylase 8; histone deacetylase-like 1; RPD3 HDAC8 CDA07, CDLS5, HD8, HDACL1, KDAC8, MRXS6, RPD3, WTS histone deacetylase 8 histone deacetylase 8|histone deacetylase-like 1

For more info on HDAC8, check out the HDAC8 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HDAC8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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