Anti-HEF1/NEDD9 Antibody Picoband™

Product Name

Anti-HEF1/NEDD9 Antibody Picoband™

View all NEDD9/CASL/HEF1 Antibodies

SKU/Catalog Number

PB9289

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-HEF1/NEDD9 Antibody Picoband™ catalog # PB9289. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HEF1/NEDD9 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9289)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human HEF1 recombinant protein (Position: K273-E421). Human HEF1 shares 83% amino acid (aa) sequence identity with mouse HEF1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9289 is reactive to NEDD9 in Human, Monkey, Mouse

Applications

PB9289 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

93-105 kDa

Calculated molecular weight

92.861kDa

Background of NEDD9/CASL/HEF1

Enhancer of filamentation 1 (EF1), also known as NEDD-9, is a protein that in humans is encoded by the NEDD9 gene. The protein encoded by this gene is a member of the CRK-associated substrates family. Members of this family are adhesion docking molecules that mediate protein-protein interactions for signal transduction pathways. It is mapped to 6p24.2. This protein is a focal adhesion protein that acts as a scaffold to regulate signaling complexes important in cell attachment, migration and invasion as well as apoptosis and the cell cycle. This protein has also been reported to have a role in cancer metastasis. NEDD9 is also a highly relevant cancer gene that governs metastatic potential in human melanoma. What’s more, it has been speculated that NEDD9 may be an important linking element between extracellular signaling and regulation of the cytoskeleton.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Monkey, Mouse

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of HEF1 using anti-HEF1 antibody (PB9289).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human Daudi whole cell lysates,
Lane 3: monkey COS-7 whole cell lysates,
Lane 4: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HEF1 antigen affinity purified polyclonal antibody (Catalog # PB9289) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HEF1 at approximately 93-105 kDa. The expected band size for HEF1 is at 93 kDa.

Gene/Protein Information For NEDD9 (Source: Uniprot.org, NCBI)

Alternative Names

Cas scaffolding protein family member 2; CAS2; CASL; CAS-LCASS2cas-like docking; CASLdJ49G10.2; CRK-associated substrate-related protein; dJ49G10.2 (Enhancer of Filamentation 1 (HEF1)); dJ761I2.1 (enhancer of filamentation (HEF1)); dJ761I2.1; enhancer of filamentation 1; HEF1; HEF1Crk-associated substrate related; NEDD9; NEDD-9; Neural precursor cell expressed developmentally down-regulated protein 9; neural precursor cell expressed, developmentally down-regulated 9; p105; Renal carcinoma antigen NY-REN-12 NEDD9 CAS-L, CAS2, CASL, CASS2, HEF1 neural precursor cell expressed, developmentally down-regulated 9 enhancer of filamentation 1|Cas scaffolding protein family member 2|Crk-associated substrate related protein Cas-L|Enhancer of filamentation 1 p55|cas-like docking|neural precursor cell expressed developmentally down-regulated protein 9|p130Cas-related protein|renal carcinoma antigen NY-REN-12

For more info on NEDD9, check out the NEDD9 Infographic

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In this infographic, you will see the following information for NEDD9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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