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Product Info Summary
| SKU: | A09185 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Helicase POLQ-like HELQ Antibody
SKU/Catalog Number
A09185
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Helicase POLQ-like HELQ Antibody catalog # A09185. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Helicase POLQ-like HELQ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09185)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant fusion protein of human HEL308(NP_598375.2).
Reactive Species
A09185 is reactive to HELQ in Human, Mouse, Rat
Calculated molecular weight
124.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09185 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000-1:3000
IHC: 1:50-1:200
Validation Images & Assay Conditions
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Western blot analysis of extracts of various cell lines, using HELQ antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.
Specific Publications For Anti-Helicase POLQ-like HELQ Antibody (A09185)
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Customer Q&As
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1 Customer Q&As for Anti-Helicase POLQ-like HELQ Antibody
Question
Can A09185 recognize PolQ helicase domain?
Verified customer
Asked: 2020-11-10
Answer
It might not be able to recognize this domain, the Anti-Helicase POLQ-like HELQ Antibody (A09185) is Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human HEL308.
Boster Scientific Support
Answered: 2020-11-12
