Anti-Hemoglobin subunit alpha HBA1 Rabbit Monoclonal Antibody

HBA1 antibody

Boster Bio Anti-Hemoglobin subunit alpha HBA1 Rabbit Monoclonal Antibody catalog # M00233. Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: M00233
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IP, IHC, WB

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Product Name

Anti-Hemoglobin subunit alpha HBA1 Rabbit Monoclonal Antibody

View all HBA1 Antibodies

SKU/Catalog Number

M00233

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Hemoglobin subunit alpha HBA1 Rabbit Monoclonal Antibody catalog # M00233. Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Hemoglobin subunit alpha HBA1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00233)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

EGI-8

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Hemoglobin subunit alpha

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00233 is reactive to HBA1 in Human, Mouse, Rat

Applications

M00233 is guaranteed for Flow Cytometry, IP, IHC, WB Boster Guarantee

Observed Molecular Weight

100-110 kDa

Calculated molecular weight

15.258kDa

Background of HBA1

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For HBA1 (Source: Uniprot.org, NCBI)

Gene Name

HBA1

Full Name

Hemoglobin subunit alpha

Weight

15.258kDa

Superfamily

globin family

Alternative Names

alpha one globin; alpha-1 globin; alpha-1-globin; alpha-globin; CD31; hemoglobin alpha 1 globin chain; Hemoglobin alpha chain; hemoglobin alpha-1 chain; hemoglobin subunit alpha; hemoglobin, alpha 1; MGC126895; MGC126897 HBA1 ECYT7, HBA-T3, HBH, METHBA hemoglobin subunit alpha 1 hemoglobin subunit alpha|alpha globin chain|alpha one globin|alpha-2 globin chain|delta globin|hemoglobin alpha 1 globin chain|hemoglobin, alpha 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on HBA1, check out the HBA1 Infographic

HBA1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HBA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00233 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Wenxia Su,Lin Yang,Xueping Luo,Meng Chen,Jinqin Liu;Elimination of Autofluorescence in Archival Formaldehyde-Fixed, Paraffin-Embedded Bone Marrow Biopsies.Arch Pathol Lab Med 1 March 2019;143(3):362–369.doi:https://doi.org/10.5858/arpa.2017-0480-OA
Species: Human
M00233 usage in article: APP:IF, SAMPLE:BONE MARROW TISSUE, DILUTION:NA

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3 Customer Q&As for Anti-Hemoglobin subunit alpha HBA1 Rabbit Monoclonal Antibody

Question

Can you help my question with product M00233, anti-Hemoglobin subunit alpha Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-04-14

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00233 anti-Hemoglobin subunit alpha Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-04-14

Question

Does M00233 anti-Hemoglobin subunit alpha Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-03-12

Answer

It shows on the product datasheet, M00233 anti-Hemoglobin subunit alpha Rabbit Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-03-12

Question

Do you have a BSA free version of anti-Hemoglobin subunit alpha Rabbit Monoclonal antibody M00233 available?

C. Anderson

Verified customer

Asked: 2013-08-20

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Hemoglobin subunit alpha Rabbit Monoclonal antibody M00233 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2013-08-20

Order DetailsPrice
M00233

100μL

$370
M00233-30ul

30μL sample

$99
M00233-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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M00233
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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