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Product Info Summary
| SKU: | M00233 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IHC, WB |
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Product info
Product Name
Anti-Hemoglobin subunit alpha HBA1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00233
BM4445 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Hemoglobin subunit alpha HBA1 Rabbit Monoclonal Antibody catalog # M00233. Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Hemoglobin subunit alpha HBA1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00233)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
EGI-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Hemoglobin subunit alpha
Reactive Species
M00233 is reactive to HBA1 in Human, Mouse, Rat
Observed Molecular Weight
15 kDa
Calculated molecular weight
15.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00233 is guaranteed for Flow Cytometry, IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: K562 cell
IHC: human spleen tissue, mouse embryo tissue
Validation Images & Assay Conditions
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Western blot analysis of Hemoglobin subunit alpha expression in K562 cell lysate.
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Immunohistochemical analysis of paraffin-embedded human spleen, using hemoglobin subunit alpha Antibody.
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Immunohistochemical analysis of paraffin-embedded mouse embryo tissue using anti-Hemoglobin subunit alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Specific Publications For Anti-Hemoglobin subunit alpha HBA1 Rabbit Monoclonal Antibody (M00233)
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3 Customer Q&As for Anti-Hemoglobin subunit alpha HBA1 Rabbit Monoclonal Antibody
Question
Can you help my question with product M00233, anti-Hemoglobin subunit alpha Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-14
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00233 anti-Hemoglobin subunit alpha Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-14
Question
Does M00233 anti-Hemoglobin subunit alpha Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
It shows on the product datasheet, M00233 anti-Hemoglobin subunit alpha Rabbit Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-12
Question
Do you have a BSA free version of anti-Hemoglobin subunit alpha Rabbit Monoclonal antibody M00233 available?
C. Anderson
Verified customer
Asked: 2013-08-20
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Hemoglobin subunit alpha Rabbit Monoclonal antibody M00233 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-08-20
