Anti-Heparan Sulfate Proteoglycan (Large) / Perlecan HSPG2 Monoclonal Antibody

Product Name

Anti-Heparan Sulfate Proteoglycan (Large) / Perlecan HSPG2 Monoclonal Antibody

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SKU/Catalog Number

M02029

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Heparan Sulfate Proteoglycan (Large) / Perlecan HSPG2 Monoclonal Antibody (Catalog # M02029). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Monkey, Cow, Pig, Mouse, Fish.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Heparan Sulfate Proteoglycan (Large) / Perlecan HSPG2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02029)

Host

Rat

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: SPM255

Isotype

IgG2a, kappa

Immunogen

Murine EHS laminin preparation

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M02029 is reactive to HSPG2 in Cow, Human, Monkey, Mouse, Pig, Fish

Applications

M02029 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee

Observed Molecular Weight

19 kDa

Calculated molecular weight

468.83kDa

Background of HSPG2

This monoclonal antibody specifically precipitates heterogeneous material of high MW, identified as perlecan, a major heparan-sulfate proteoglycan (HSPG) within all basement membranes and cell surfaces. It does not cross-react with laminin, fibronectin, or dermatran sulfate proteoglycan. Because of perlecan's strategic location and ability to store and protect growth factors, it has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a co-receptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Anti-Heparan Sulfate Monoclonal Antibody (SPM255).

Gene/Protein Information For HSPG2 (Source: Uniprot.org, NCBI)

Alternative Names

basement membrane-specific heparan sulfate proteoglycan core protein; endorepellin (domain V region); Endorepellin; heparan sulfate proteoglycan 2; HSPG; HSPG2; perlecan proteoglycan; Perlecan; PLCSchwartz-Jampel syndrome 1 (chondrodystrophic myotonia); PRCAN; SJA; SJS; SJS1 HSPG2 HSPG, PLC, PRCAN, SJA, SJS, SJS1 heparan sulfate proteoglycan 2 basement membrane-specific heparan sulfate proteoglycan core protein|endorepellin (domain V region)|perlecan proteoglycan

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In this infographic, you will see the following information for HSPG2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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