Product Info Summary
SKU: | M01459 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Hes1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01459
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Hes1 Rabbit Monoclonal Antibody catalog # M01459. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Hes1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01459)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
FBB-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Hes1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M01459 is reactive to HES1 in Human, Mouse, Rat
Applications
M01459 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
30 kDa
Calculated molecular weight
29.541kDa
Background of HES-1
Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Hes1 using anti-Hes1 antibody (M01459).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hes1 antigen affinity purified monoclonal antibody (Catalog # M01459) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hes1 at approximately 30 kDa. The expected band size for Hes1 is at 30 kDa.
Click image to see more details
IHC analysis of Hes1 using anti-Hes1 antibody (M01459) on human liver.
Hes1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Hes1 Antibody (M01459) overnight at 4°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Protein Target Info & Infographic
Gene/Protein Information For HES1 (Source: Uniprot.org, NCBI)
Gene Name
HES1
Full Name
Transcription factor HES-1
Weight
29.541kDa
Alternative Names
BHLHB39; bHLHb39hairy homolog (Drosophila); Class B basic helix-loop-helix protein 39; FLJ20408; Hairy and enhancer of split 1; hairy and enhancer of split 1, (Drosophila); Hairy homolog; Hairy-like protein; HES1; HES-1; hHL; HL; HRY; HRYHHL; transcription factor HES-1 HES1 HES-1, HHL, HRY, bHLHb39 hes family bHLH transcription factor 1 transcription factor HES-1|class B basic helix-loop-helix protein 39|hairy homolog|hairy-like protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HES1, check out the HES1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HES1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hes1 Rabbit Monoclonal Antibody (M01459)
Hello CJ!
M01459 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Shi J,Han G,Wang J,Han X,Zhao M,Duan X,Mi L,Li N,Yin X,Shi H,Li C,Xu J,Yin F.Matrine promotes hepatic oval cells differentiation into hepatocytes and alleviates liver injury by suppression of Notch signalling pathway.Life Sci.2020 Nov 15;261:118354.doi:10.1016/j.lfs.2020.118354.Epub 2020 Aug 28.PMID:32866517.
Species: Human,Rat
M01459 usage in article: APP:WB, SAMPLE:HOCS, DILUTION:1:1000
Qiu M, Bao W, Wang J, Yang T, He X, Liao Y, Wan X. Bmc Cancer. 2014 Feb 11;14:78. Doi: 10.1186/1471-2407-14-78. Foxa1 Promotes Tumor Cell Proliferation Through Ar Involving The Notch Pathway In Endometrial Cancer.
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Hes1 Rabbit Monoclonal Antibody
Question
We are currently using anti-Hes1 Rabbit Monoclonal antibody M01459 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
The anti-Hes1 Rabbit Monoclonal antibody (M01459) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-05
Question
Here is the WB image, lot number and protocol we used for erythroleukemia using anti-Hes1 Rabbit Monoclonal antibody M01459. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-26
Question
Is this M01459 anti-Hes1 Rabbit Monoclonal antibody reactive to the isotypes of HES1?
Verified Customer
Verified customer
Asked: 2018-08-09
Answer
The immunogen of M01459 anti-Hes1 Rabbit Monoclonal antibody is A synthesized peptide derived from human Hes1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-08-09
Question
Is a blocking peptide available for product anti-Hes1 Rabbit Monoclonal antibody (M01459)?
N. Wu
Verified customer
Asked: 2015-08-07
Answer
We do provide the blocking peptide for product anti-Hes1 Rabbit Monoclonal antibody (M01459). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-08-07