Anti-Hes1 Rabbit Monoclonal Antibody

HES-1 antibody

Boster Bio Anti-Hes1 Rabbit Monoclonal Antibody catalog # M01459. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: M01459
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-Hes1 Rabbit Monoclonal Antibody

View all HES-1 Antibodies

SKU/Catalog Number

M01459

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Hes1 Rabbit Monoclonal Antibody catalog # M01459. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Hes1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01459)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

FBB-8

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Hes1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M01459 is reactive to HES1 in Human, Mouse, Rat

Applications

M01459 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

30 kDa

Calculated molecular weight

29.541kDa

Background of HES-1

Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For HES1 (Source: Uniprot.org, NCBI)

Gene Name

HES1

Full Name

Transcription factor HES-1

Weight

29.541kDa

Alternative Names

BHLHB39; bHLHb39hairy homolog (Drosophila); Class B basic helix-loop-helix protein 39; FLJ20408; Hairy and enhancer of split 1; hairy and enhancer of split 1, (Drosophila); Hairy homolog; Hairy-like protein; HES1; HES-1; hHL; HL; HRY; HRYHHL; transcription factor HES-1 HES1 HES-1, HHL, HRY, bHLHb39 hes family bHLH transcription factor 1 transcription factor HES-1|class B basic helix-loop-helix protein 39|hairy homolog|hairy-like protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on HES1, check out the HES1 Infographic

HES1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HES1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M01459 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Shi J,Han G,Wang J,Han X,Zhao M,Duan X,Mi L,Li N,Yin X,Shi H,Li C,Xu J,Yin F.Matrine promotes hepatic oval cells differentiation into hepatocytes and alleviates liver injury by suppression of Notch signalling pathway.Life Sci.2020 Nov 15;261:118354.doi:10.1016/j.lfs.2020.118354.Epub 2020 Aug 28.PMID:32866517.
Species: Human,Rat
M01459 usage in article: APP:WB, SAMPLE:HOCS, DILUTION:1:1000

Qiu M, Bao W, Wang J, Yang T, He X, Liao Y, Wan X. Bmc Cancer. 2014 Feb 11;14:78. Doi: 10.1186/1471-2407-14-78. Foxa1 Promotes Tumor Cell Proliferation Through Ar Involving The Notch Pathway In Endometrial Cancer.

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4 Customer Q&As for Anti-Hes1 Rabbit Monoclonal Antibody

Question

We are currently using anti-Hes1 Rabbit Monoclonal antibody M01459 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2019-11-05

Answer

The anti-Hes1 Rabbit Monoclonal antibody (M01459) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-11-05

Question

Here is the WB image, lot number and protocol we used for erythroleukemia using anti-Hes1 Rabbit Monoclonal antibody M01459. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-06-26

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-26

Question

Is this M01459 anti-Hes1 Rabbit Monoclonal antibody reactive to the isotypes of HES1?

Verified Customer

Verified customer

Asked: 2018-08-09

Answer

The immunogen of M01459 anti-Hes1 Rabbit Monoclonal antibody is A synthesized peptide derived from human Hes1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-08-09

Question

Is a blocking peptide available for product anti-Hes1 Rabbit Monoclonal antibody (M01459)?

N. Wu

Verified customer

Asked: 2015-08-07

Answer

We do provide the blocking peptide for product anti-Hes1 Rabbit Monoclonal antibody (M01459). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2015-08-07

Order DetailsPrice
M01459

100μL

$370
M01459-30ul

30μL sample

$99
M01459-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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M01459
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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