Anti-HEXA Antibody

Hexosaminidase A/HEXA antibody

Boster Bio Anti-HEXA Antibody catalog # PA1787-2. Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: PA1787-2
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

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Product Name

Anti-HEXA Antibody

View all Hexosaminidase A/HEXA Antibodies

SKU/Catalog Number

PA1787-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-HEXA Antibody catalog # PA1787-2. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HEXA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1787-2)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human HEXA, different from the related mouse sequence by three amino acids, and from the related rat sequences by four amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1787-2 is reactive to HEXA in Human

Applications

PA1787-2 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

57 kDa

Calculated molecular weight

60.703kDa

Background of Hexosaminidase A/HEXA

HEXA (hexosaminidase A (alpha polypeptide)) is an enzyme that in humans is encoded by the HEXA gene. Hexosaminidase A and the cofactor GM2 activator protein catalyze the degradation of the GM2 gangliosides and other molecules containing terminal N-acetyl hexosamines The HEXA gene encodes the alpha subunit of hexosaminidase A, a lysosomal enzyme involved in the breakdown of gangliosides. The HEXA gene is mapped on 15q23. Even though the alpha and beta subunits of hexosaminidase A can both cleave GalNAc residues, only the alpha subunit is able to hydrolyze GM2 gangliosides. The alpha subunit contains a key residue, Arg-424, which is essential for binding the N-acetyl-neuramanic residue of GM2 gangliosides. Chimeric constructs were expressed in HeLa cells and selected constructs were produced in the baculovirus expression system to determine their ability to degrade GM2 ganglioside in the presence of GM2 activator protein. Their results allowed them to define 2 noncontiguous sequences in the alpha subunit (amino acids 1-191 and 403-529) which, when substituted into analogous positions in the beta subunit, conferred activity against the sulfated substrate.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For HEXA (Source: Uniprot.org, NCBI)

Gene Name

HEXA

Full Name

Beta-hexosaminidase subunit alpha

Weight

60.703kDa

Superfamily

glycosyl hydrolase 20 family

Alternative Names

beta-hexosaminidase subunit alpha; Beta-N-acetylhexosaminidase subunit alpha; EC 3.2.1; EC 3.2.1.52; HEXA; hexosaminidase A (alpha polypeptide); Hexosaminidase A; Hexosaminidase subunit A; MGC99608; N-acetyl-beta-glucosaminidase subunit alpha; TSD HEXA TSD hexosaminidase subunit alpha beta-hexosaminidase subunit alpha|N-acetyl-beta-glucosaminidase subunit alpha|beta-N-acetylhexosaminidase subunit alpha|hexosaminidase A (alpha polypeptide)|hexosaminidase subunit A

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on HEXA, check out the HEXA Infographic

HEXA infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HEXA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-HEXA Antibody

Question

Will anti-HEXA antibody PA1787-2 work for WB with thalamus?

Verified Customer

Verified customer

Asked: 2020-05-05

Answer

According to the expression profile of thalamus, HEXA is highly expressed in thalamus. So, it is likely that anti-HEXA antibody PA1787-2 will work for WB with thalamus.

Boster Scientific Support

Answered: 2020-05-05

Question

Does anti-HEXA antibody PA1787-2 work on zebrafish WB with thalamus?

Verified Customer

Verified customer

Asked: 2020-04-15

Answer

Our lab technicians have not tested anti-HEXA antibody PA1787-2 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-HEXA antibody PA1787-2 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish thalamus in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-04-15

Question

Would PA1787-2 anti-HEXA antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-03-03

Answer

You can see on the product datasheet, PA1787-2 anti-HEXA antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-03-03

Question

Is a blocking peptide available for product anti-HEXA antibody (PA1787-2)?

Verified Customer

Verified customer

Asked: 2019-07-26

Answer

We do provide the blocking peptide for product anti-HEXA antibody (PA1787-2). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-07-26

Question

I was wanting to use your anti-HEXA antibody for WB for human thalamus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human thalamus identification?

Verified Customer

Verified customer

Asked: 2019-05-27

Answer

You can see on the product datasheet, PA1787-2 anti-HEXA antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human thalamus in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-27

Question

We are currently using anti-HEXA antibody PA1787-2 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?

A. Singh

Verified customer

Asked: 2019-04-29

Answer

The anti-HEXA antibody (PA1787-2) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-04-29

Order DetailsPrice
PA1787-2

100μg

$370
PA1787-2-10ug

10μg sample (liquid)

$99
PA1787-2-Biotin

100 μg Biotin conjugated

$570
PA1787-2-Cy3

100 μg Cy3 conjugated

$570
PA1787-2-Dylight488

100 μg Dylight488 conjugated

$570
PA1787-2-Dylight550

100 μg Dylight550 conjugated

$570
PA1787-2-Dylight594

100 μg Dylight594 conjugated

$570
PA1787-2-FITC

100 μg FITC conjugated

$570
PA1787-2-HRP

100 μg HRP conjugated

$570
PA1787-2-APC

100 μg APC conjugated

$670
PA1787-2-PE

100 μg PE conjugated

$670
PA1787-2-carrier-free

Carrier Free

$370

More conjugates/formats

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PA1787-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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