Click image to see more details
Product Info Summary
| SKU: | PA1787-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-HEXA Antibody Picoband®
SKU/Catalog Number
PA1787-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HEXA Antibody catalog # PA1787-2. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HEXA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1787-2)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human HEXA, different from the related mouse sequence by three amino acids, and from the related rat sequences by four amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1787-2 is reactive to HEXA in Human
Observed Molecular Weight
57 kDa
Calculated molecular weight
60.7 kDa
Background of HEXA
HEXA (hexosaminidase A (alpha polypeptide)) is an enzyme that in humans is encoded by the HEXA gene. Hexosaminidase A and the cofactor GM2 activator protein catalyze the degradation of the GM2 gangliosides and other molecules containing terminal N-acetyl hexosamines The HEXA gene encodes the alpha subunit of hexosaminidase A, a lysosomal enzyme involved in the breakdown of gangliosides. The HEXA gene is mapped on 15q23. Even though the alpha and beta subunits of hexosaminidase A can both cleave GalNAc residues, only the alpha subunit is able to hydrolyze GM2 gangliosides. The alpha subunit contains a key residue, Arg-424, which is essential for binding the N-acetyl-neuramanic residue of GM2 gangliosides. Chimeric constructs were expressed in HeLa cells and selected constructs were produced in the baculovirus expression system to determine their ability to degrade GM2 ganglioside in the presence of GM2 activator protein. Their results allowed them to define 2 noncontiguous sequences in the alpha subunit (amino acids 1-191 and 403-529) which, when substituted into analogous positions in the beta subunit, conferred activity against the sulfated substrate.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1787-2 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human Hela whole cell, human 293T whole cell, human Caco-2 whole cell, human THP-1 whole cell, human HepG2 whole cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of HEXA using anti-HEXA antibody (PA1787-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human THP-1 whole cell lysates,
Lane 5: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HEXA antigen affinity purified polyclonal antibody (Catalog # PA1787-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HEXA at approximately 57 kDa. The expected band size for HEXA is at 60 kDa.
Specific Publications For Anti-HEXA Antibody Picoband® (PA1787-2)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-HEXA Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-HEXA Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Anti-HEXA Antibody Picoband®
Question
Will anti-HEXA antibody PA1787-2 work for WB with thalamus?
Verified Customer
Verified customer
Asked: 2020-05-05
Answer
According to the expression profile of thalamus, HEXA is highly expressed in thalamus. So, it is likely that anti-HEXA antibody PA1787-2 will work for WB with thalamus.
Boster Scientific Support
Answered: 2020-05-05
Question
Does anti-HEXA antibody PA1787-2 work on zebrafish WB with thalamus?
Verified Customer
Verified customer
Asked: 2020-04-15
Answer
Our lab technicians have not tested anti-HEXA antibody PA1787-2 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-HEXA antibody PA1787-2 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish thalamus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-15
Question
Would PA1787-2 anti-HEXA antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
You can see on the product datasheet, PA1787-2 anti-HEXA antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-03
Question
Is a blocking peptide available for product anti-HEXA antibody (PA1787-2)?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
We do provide the blocking peptide for product anti-HEXA antibody (PA1787-2). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-07-26
Question
I was wanting to use your anti-HEXA antibody for WB for human thalamus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human thalamus identification?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
You can see on the product datasheet, PA1787-2 anti-HEXA antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human thalamus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-27
Question
We are currently using anti-HEXA antibody PA1787-2 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?
A. Singh
Verified customer
Asked: 2019-04-29
Answer
The anti-HEXA antibody (PA1787-2) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-29
