Anti-HEXB Antibody Picoband™

Product Name

Anti-HEXB Antibody Picoband™

View all HEXB Antibodies

SKU/Catalog Number

PB9211

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-HEXB Antibody Picoband™ catalog # PB9211. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HEXB Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9211)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human HEXB recombinant protein (Position: K381-M556). Human HEXB shares 75% and 73% amino acid (aa) sequences identity with mouse and rat HEXB, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9211 is reactive to HEXB in Human

Applications

PB9211 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

63 kDa

Calculated molecular weight

63.111kDa

Background of HEXB

Beta-hexosaminidase subunit beta (HEXB) is an enzyme that in humans is encoded by the HEXB gene. It is mapped to 5q13.3. HEXB is the beta subunit of the lysosomal enzyme beta-hexosaminidase that, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Beta subunit gene mutations lead to Sandhoff disease (GM2-gangliosidosis type II). It has been found that HEXB is a peptidoglycan hydrolase and it is involved in restricting mycobacteria growth even before the onset of adaptive immunity.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of HEXB using anti-HEXB antibody (PB9211).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U87 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HEXB antigen affinity purified polyclonal antibody (Catalog # PB9211) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HEXB at approximately 63 kDa. The expected band size for HEXB is at 63 kDa.

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Anti-HEXB antibody, PB9211,IHC(P)
IHC(P): Human Lung Cancer Tissue

Gene/Protein Information For HEXB (Source: Uniprot.org, NCBI)

Alternative Names

beta-hexosaminidase subunit beta; Beta-N-acetylhexosaminidase subunit beta; Cervical cancer proto-oncogene 7 protein; EC 3.2.1.52; ENC-1AS; HCC-7; HEL-248; HEXB; hexosaminidase B (beta polypeptide); Hexosaminidase B; Hexosaminidase subunit B; N-acetyl-beta-glucosaminidase subunit beta HEXB ENC-1AS, HEL-248, HEL-S-111 hexosaminidase subunit beta beta-hexosaminidase subunit beta|HCC-7|N-acetyl-beta-glucosaminidase subunit beta|beta-N-acetylhexosaminidase subunit beta|beta-hexosaminidase beta-subunit|cervical cancer proto-oncogene 7 protein|epididymis luminal protein 248|epididymis secretory protein Li 111|epididymis secretory sperm binding protein|hexosaminidase B (beta polypeptide)|hexosaminidase subunit B

For more info on HEXB, check out the HEXB Infographic

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In this infographic, you will see the following information for HEXB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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