Anti-Hexb (S516) Antibody

Product Name

Anti-Hexb (S516) Antibody

View all HEXB Antibodies

SKU/Catalog Number

A02290S516

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Hexb (S516) Antibody catalog # A02290S516. Tested in WB,IHC applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Hexb (S516) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02290S516)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human keratin 17.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02290S516 is reactive to HEXB in Human

Applications

A02290S516 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

63.111kDa

Background of HEXB

May play a role in the formation and maintenance of various skin appendages, specifically in determining shape and orientation of hair. May be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial "stem cells". May act as an autoantigen in the immunopathogenesis of psoriasis, with certain peptide regions being a major target for autoreactive T-cells and hence causing their proliferation. Required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state. Modulates the function of TNF-alpha in the specific context of hair cycling. Regulates protein synthesis and epithelial cell growth through binding to the adapter protein SFN and by stimulating Akt/mTOR pathway. Involved in tissue repair By similarity.

M Rosenberg, Mol. Cell. Biol., Feb 1988; 8: 722 - 736.
SM Troyanovsky, J. Cell Sci., Jul 1989; 93: 419.
Kevin M. McGowan, Genes & Dev., Jun 2002; 16: 1412.
Nicholas Bianchi, Mol. Cell. Biol., Aug 2005; 25: 7249 - 7259.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:1000
IHC: 1:50-1:200

Validation Images & Assay Conditions

Click image to see more details

Figure 2. Immunohistochemistry validation of HEXB using Anti-Hexb (S516) Antibody (A02290S516).

Immunohistochemistry (IHC) analyzes of Hexb (S516) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
For more protocol information of IHC

Click image to see more details

Figure 1. Western blotting validation for Anti-Hexb (S516) Antibody A02290S516

Western blot (WB) analysis of Hexb (S516) pAb at 1:500 dilution
Lane1:A549 whole cell lysate(40ug)
Lane2:MCF-7 whole cell lysate(40ug)
Lane3:U-87MG whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For HEXB (Source: Uniprot.org, NCBI)

Alternative Names

beta-hexosaminidase subunit beta; Beta-N-acetylhexosaminidase subunit beta; Cervical cancer proto-oncogene 7 protein; EC 3.2.1.52; ENC-1AS; HCC-7; HEL-248; HEXB; hexosaminidase B (beta polypeptide); Hexosaminidase B; Hexosaminidase subunit B; N-acetyl-beta-glucosaminidase subunit beta HEXB ENC-1AS, HEL-248, HEL-S-111 hexosaminidase subunit beta beta-hexosaminidase subunit beta|HCC-7|N-acetyl-beta-glucosaminidase subunit beta|beta-N-acetylhexosaminidase subunit beta|beta-hexosaminidase beta-subunit|cervical cancer proto-oncogene 7 protein|epididymis luminal protein 248|epididymis secretory protein Li 111|epididymis secretory sperm binding protein|hexosaminidase B (beta polypeptide)|hexosaminidase subunit B

For more info on HEXB, check out the HEXB Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HEXB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the HEXB Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis