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Product Info Summary
| SKU: | A01504-2 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Hexokinase 1 HK1 Antibody
View all Hexokinase 1 Antibodies
SKU/Catalog Number
A01504-2
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hexokinase 1 HK1 Antibody (Catalog # A01504-2). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Hexokinase 1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Hexokinase 1 HK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01504-2)
Host
Rabbit
Contents
Hexokinase 1 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
Hexokinase 1 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human Hexokinase 1. The immunogen is located within amino acids 830 - 880 of Hexokinase 1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Hexokinase 1 antibody is human, mouse and rat reactive. Multiple isoforms of Hexokinase 1 are known to exist.
Reactive Species
A01504-2 is reactive to HK1 in Human, Mouse, Rat
Applications
A01504-2 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
102.486kDa
Background of Hexokinase 1
There are four major glucose-phosphorylating isoenzymes, designated Hexokinase 1 I, II, III, and IV (1). Hexokinase 1 activity is involved in the first step in several metabolic pathways including phosphorylation of glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway (1,2). Hexokinase 1 2 is the predominant Hexokinase 1 isozyme expressed in insulin-responsive tissues such as skeletal muscle and its expression is insulin-responsive (3). It is involved in the increased rate of glycolysis seen in rapidly growing cancer cells (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Hexokinase 1 antibody can be used for detection of Hexokinase 1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Hexokinase 1 in rat bladder tissue lysate with Hexokinase 1 antibody at (A) 1 and (B) 2 μg/ml.
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Immunohistochemistry of Hexokinase 1 in rat stomach tissue with Hexokinase 1 antibody at 5 μg/mL.
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Immunofluorescence of Hexokinase 1 in rat stomach tissue with Hexokinase 1 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For HK1 (Source: Uniprot.org, NCBI)
Gene Name
HK1
Full Name
Hexokinase-1
Weight
102.486kDa
Superfamily
hexokinase family
Alternative Names
Brain form hexokinase; EC 2.7.1; EC 2.7.1.1; glycolytic enzyme; Hexokinase 1; Hexokinase type I; hexokinase-1; HK I; HK1; HK1-ta; HK1-tb; HK1-tc; HKI; HXK1 HK1 HK-ta, HK1-tb, HK1-tc, HKD, HKI, HMSNR, HXK1, NEDVIBA, RP79, hexokinase, HK1 hexokinase 1 hexokinase-1|brain form hexokinase|glycolytic enzyme|hexokinase IR|hexokinase type I|hexokinase-A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HK1, check out the HK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hexokinase 1 HK1 Antibody (A01504-2)
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Customer Reviews
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