Product Info Summary
SKU: | A01504-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Hexokinase 1/HK1 Antibody Picoband™
View all Hexokinase 1 Antibodies
SKU/Catalog Number
A01504-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Hexokinase 1/HK1 Antibody Picoband™ catalog # A01504-1. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Hexokinase 1/HK1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01504-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Hexokinase 1/HK1 recombinant protein (Position: D17-R323).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01504-1 is reactive to HK1 in Human, Mouse, Rat
Applications
A01504-1 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
102 kDa
Calculated molecular weight
102.486kDa
Background of Hexokinase 1
Hexokinase-1 (HK1) is an enzyme that in humans is encoded by the HK1 gene on chromosome 10. It is mapped to 10q22.1. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Direct ELISA, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Hexokinase 1/HK1 using anti-Hexokinase 1/HK1 antibody (A01504-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human U20S whole cell lysates,
Lane 3: human Hek293 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat heart tissue lysates,
Lane 7: rat lung tissue lysates,
Lane 8: mouse brain tissue lysates,
Lane 9: mouse heart tissue lysates,
Lane 10: mouse lung tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hexokinase 1/HK1 antigen affinity purified polyclonal antibody (Catalog # A01504-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hexokinase 1/HK1 at approximately 102KD. The expected band size for Hexokinase 1/HK1 is at 102KD.
Click image to see more details
Figure 2. IHC analysis of Hexokinase 1/HK1 using anti-Hexokinase 1/HK1 antibody (A01504-1).
Hexokinase 1/HK1 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Hexokinase 1/HK1 Antibody (A01504-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Hexokinase 1/HK1 using anti-Hexokinase 1/HK1 antibody (A01504-1).
Hexokinase 1/HK1 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Hexokinase 1/HK1 Antibody (A01504-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of Hexokinase 1/HK1 using anti-Hexokinase 1/HK1 antibody (A01504-1).
Hexokinase 1/HK1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Hexokinase 1/HK1 Antibody (A01504-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of Hexokinase 1/HK1 using anti-Hexokinase 1/HK1 antibody (A01504-1).
Hexokinase 1/HK1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Hexokinase 1/HK1 Antibody (A01504-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 6. IF analysis of Hexokinase 1/HK1 using anti-Hexokinase 1/HK1 antibody (A01504-1).
Hexokinase 1/HK1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Hexokinase 1/HK1 Antibody (A01504-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For HK1 (Source: Uniprot.org, NCBI)
Gene Name
HK1
Full Name
Hexokinase-1
Weight
102.486kDa
Superfamily
hexokinase family
Alternative Names
Brain form hexokinase; EC 2.7.1; EC 2.7.1.1; glycolytic enzyme; Hexokinase 1; Hexokinase type I; hexokinase-1; HK I; HK1; HK1-ta; HK1-tb; HK1-tc; HKI; HXK1 HK1 HK-ta, HK1-tb, HK1-tc, HKD, HKI, HMSNR, HXK1, NEDVIBA, RP79, hexokinase, HK1 hexokinase 1 hexokinase-1|brain form hexokinase|glycolytic enzyme|hexokinase IR|hexokinase type I|hexokinase-A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HK1, check out the HK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hexokinase 1/HK1 Antibody Picoband™ (A01504-1)
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