Anti-hHR23A/RAD23A Antibody Picoband™

Product Name

Anti-hHR23A/RAD23A Antibody Picoband™

View all Rad23 Antibodies

SKU/Catalog Number

A03243-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-hHR23A/RAD23A Antibody Picoband™ catalog # A03243-3. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-hHR23A/RAD23A Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03243-3)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human hHR23A/RAD23A, identical to the related mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03243-3 is reactive to RAD23A in Human, Mouse, Rat

Applications

A03243-3 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

60 kDa

Calculated molecular weight

39.609kDa

Background of Rad23

UV excision repair protein RAD23 homolog A is a protein that in humans is encoded by the RAD23A gene. The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in nucleotide excision repair. Proteins in this family have a modular domain structure consisting of an ubiquitin-like domain (UbL), ubiquitin-associated domain 1 (UbA1), XPC-binding domain and UbA2. The protein encoded by this gene plays an important role in nucleotide excision repair and also in delivery of polyubiquitinated proteins to the proteasome. Alternative splicing results in multiple transcript variants encoding multiple isoforms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3 μg/1x10⁶ cells, Human, Mouse, Rat

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of HHR23A/RAD23A using anti-HHR23A/RAD23A antibody (A03243-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: rat testis tissue lysates,
Lane 6: mouse testis tissue lysates,
Lane 7: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HHR23A/RAD23A antigen affinity purified polyclonal antibody (Catalog # A03243-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HHR23A/RAD23A at approximately 60 kDa. The expected band size for HHR23A/RAD23A is at 60 kDa.

Click image to see more details

Figure 2. Flow Cytometry analysis of ANA-1 cells using anti-HHR23A/RAD23A antibody (A03243-3).
Overlay histogram showing ANA-1 cells stained with A03243-3 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HHR23A/RAD23A Antibody (A03243-3, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Click image to see more details

Figure 3. Flow Cytometry analysis of C6 cells using anti-HHR23A/RAD23A antibody (A03243-3).
Overlay histogram showing C6 cells stained with A03243-3 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HHR23A/RAD23A Antibody (A03243-3, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Click image to see more details

Figure 4. Flow Cytometry analysis of Hela cells using anti-HHR23A/RAD23A antibody (A03243-3).
Overlay histogram showing Hela cells stained with A03243-3 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HHR23A/RAD23A Antibody (A03243-3, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For RAD23A (Source: Uniprot.org, NCBI)

Alternative Names

hHR23; Rad23 RAD23A HHR23A, HR23A RAD23 homolog A, nucleotide excision repair protein UV excision repair protein RAD23 homolog A|RAD23, yeast homolog, A

For more info on RAD23A, check out the RAD23A Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for RAD23A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the RAD23A Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis