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Product Info Summary
| SKU: | A04630-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Monkey |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-HIC5/TGFB1I1 Antibody Picoband®
SKU/Catalog Number
A04630-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HIC5/TGFB1I1 Antibody Picoband® catalog # A04630-2. Tested in ELISA, WB applications. This antibody reacts with Human, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-HIC5/TGFB1I1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04630-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human HIC5/TGFB1I1 recombinant protein (Position: E268-A447).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04630-2 is reactive to TGFB1I1 in Human, Monkey
Observed Molecular Weight
75 kDa
Calculated molecular weight
49.8 kDa
Background of TGFB1I1
Transforming growth factor beta-1-induced transcript 1 protein is a protein that in humans is encoded by the TGFB1I1 gene. This gene encodes a coactivator of the androgen receptor, a transcription factor which is activated by androgen and has a key role in male sexual differentiation. The encoded protein is thought to regulate androgen receptor activity and may have a role to play in the treatment of prostate cancer. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04630-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Monkey
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: monkey COS-7 whole cell
Validation Images & Assay Conditions
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Western blot analysis of HIC5/TGFB1I1 using anti-HIC5/TGFB1I1 antibody (A04630-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: monkey COS-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIC5/TGFB1I1 antigen affinity purified polyclonal antibody (Catalog # A04630-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIC5/TGFB1I1 at approximately 75 kDa. The expected band size for HIC5/TGFB1I1 is at 75 kDa.
Specific Publications For Anti-HIC5/TGFB1I1 Antibody Picoband® (A04630-2)
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