Anti-Hif3 alpha Antibody

Product Name

Anti-Hif3 alpha Antibody

View all HIF-3 alpha Antibodies

SKU/Catalog Number

A03805-1

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Hif3 alpha Antibody (Catalog # A03805-1). Tested in ELISA, WB applications. This antibody reacts with Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Hif3 alpha Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03805-1)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 575-600 of mouse Hif3 a (Hypoxia Inducible Factor).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A03805-1 is reactive to Hif3a in Mouse

Applications

A03805-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

73.044kDa

Background of HIF-3 alpha

One of the most important factors in the cellular response to hypoxia is hypoxia-inducible factor (HIF), which transcriptionally activates genes encoding proteins that mediate adaptive responses to reduced oxygen availability. HIF is a member of the basic helix-loop-helix (bHLH) superfamily, in which the HLH domain mediates subunit dimerization while the basic domain binds to DNA. HIF binds to the hypoxia-responsive elements (HRE) located within the enhancer/promoter of hypoxia-inducible target genes and hence inhibits HRE-driven transcriptional activation. HIF target genes play critical roles in metabolism, angiogenesis, cell proliferation, and cell survival; in fact, HIF3-a may be a marker for tumor growth and apoptosis. Hif3 may participate in disorders with the cornea, lung, and heart. Anti-Hif3 alpha Antibody is useful for researchers interested in transcription factors, DNA binding, and Epilepsy research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:14,000 - 1:80,000
WB: 1:1,000 - 1:8,000

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Hif3a using anti-Hif3a antibody (A03805-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hif3a antigen affinity purified polyclonal antibody (Catalog # A03805-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for Hif3a.

Gene/Protein Information For Hif3a (Source: Uniprot.org, NCBI)

Alternative Names

Basic-helix-loop-helix-PAS protein MOP7; BHLHE17; bHLHe17HIF-3A2; Class E basic helix-loop-helix protein 17; HIF-3A4; HIF-3-alpha; HIF3-alpha; hypoxia inducible factor 3, alpha subunit; hypoxia-inducible factor 3-alpha; hypoxia-inducible factor-3 alpha 4; Inhibitory PAS domain protein; IPASHIF3-alpha-1; Member of PAS protein 7; MOP7PAS domain-containing protein 7; PASD7HIF-3A Hif3a|Ipas, MO, MOP7, N, NEPAS, bHLHe1, bHLHe17|hypoxia inducible factor 3, alpha subunit|hypoxia-inducible factor 3-alpha|HIF-3-alpha|HIF3-alpha|HIF3-alpha-1|basic-helix-loop-helix-PAS protein MOP7|hypoxia inducible factor three alpha|inhibitory PAS domain protein|member of PAS protein 7|neonatal and embryonic PAS protein

For more info on Hif3a, check out the Hif3a Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Hif3a: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the Hif3a Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis