Anti-HIP1 Monoclonal Antibody

Product Name

Anti-HIP1 Monoclonal Antibody

View all HIP1 Antibodies

SKU/Catalog Number

M02242

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-HIP1 Monoclonal Antibody catalog # M02242. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HIP1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02242)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AFG-8

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human HIP1 Plays a role in clathrin-mediated endocytosis and trafficking. Involved in regulating AMPA receptor trafficking in the central nervous system in an NMDA-dependent manner. Enhances androgen receptor (AR) -mediated transcription.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M02242 is reactive to HIP1 in Human, Mouse, Rat

Applications

M02242 is guaranteed for IP, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

116 kDa

Calculated molecular weight

116.221kDa

Background of HIP1

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:30

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of HIP1 using anti-HIP1 antibody (M02242).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIP1 antigen affinity purified monoclonal antibody (Catalog # M02242) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIP1 at approximately 116 kDa. The expected band size for HIP1 is at 116 kDa.

Gene/Protein Information For HIP1 (Source: Uniprot.org, NCBI)

Alternative Names

HIP-1; HIP-I; huntingtin interacting protein 1; huntingtin-interacting protein 1; Huntingtin-interacting protein I; ILWEQ; MGC126506 HIP1 HIP-I, ILWEQ, SHON, SHONbeta, SHONgamma huntingtin interacting protein 1 huntingtin-interacting protein 1|huntingtin-interacting protein I

For more info on HIP1, check out the HIP1 Infographic

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In this infographic, you will see the following information for HIP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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