Click image to see more details
Product Info Summary
| SKU: | M02242 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-HIP1 Monoclonal Antibody
SKU/Catalog Number
M02242
BM5424 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-HIP1 Monoclonal Antibody catalog # M02242. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HIP1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02242)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AFG-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human HIP1 Plays a role in clathrin-mediated endocytosis and trafficking. Involved in regulating AMPA receptor trafficking in the central nervous system in an NMDA-dependent manner. Enhances androgen receptor (AR) -mediated transcription.
Reactive Species
M02242 is reactive to HIP1 in Human, Mouse, Rat
Observed Molecular Weight
116 kDa
Calculated molecular weight
116.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02242 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
IP 1:30
Positive Control
WB: human A549 whole cell, human MCF-7 whole cell, human RT-4 whole cell, human SH-SY5Y whole cell, rat brain tissue,mouse brain tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of HIP1 using anti-HIP1 antibody (M00346-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human RT4 whole cell lysates,
Lane 4: human SH-SY5Y whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIP1 antigen affinity purified monoclonal antibody (M00346-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIP1 at approximately 116 kDa. The expected band size for HIP1 is at 116 kDa.
Specific Publications For Anti-HIP1 Monoclonal Antibody (M02242)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-HIP1 Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-HIP1 Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Anti-HIP1 Monoclonal Antibody
Question
We are currently using anti-HIP1 Monoclonal antibody M02242 for rat tissue, and we are happy with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
The anti-HIP1 Monoclonal antibody (M02242) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-25
Question
Is a blocking peptide available for product anti-HIP1 Monoclonal antibody (M02242)?
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
We do provide the blocking peptide for product anti-HIP1 Monoclonal antibody (M02242). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-07-18
Question
Will anti-HIP1 Monoclonal antibody M02242 work for IF with corpus callosum?
C. Roberts
Verified customer
Asked: 2019-06-27
Answer
According to the expression profile of corpus callosum, HIP1 is highly expressed in corpus callosum. So, it is likely that anti-HIP1 Monoclonal antibody M02242 will work for IF with corpus callosum.
Boster Scientific Support
Answered: 2019-06-27
Question
Will M02242 anti-HIP1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-08-28
Answer
As indicated on the product datasheet, M02242 anti-HIP1 Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-08-28
Question
Is this M02242 anti-HIP1 Monoclonal antibody reactive to the isotypes of HIP1?
D. Collins
Verified customer
Asked: 2013-12-06
Answer
The immunogen of M02242 anti-HIP1 Monoclonal antibody is A synthesized peptide derived from human HIP1 Plays a role in clathrin-mediated endocytosis and trafficking. Involved in regulating AMPA receptor trafficking in the central nervous system in an NMDA-dependent manner. Enhances androgen receptor (AR) -mediated transcription. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-12-06
