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Product Info Summary
| SKU: | A05895-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, IHC-P, WB |
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Product info
Product Name
Anti-HIP2 Antibody (Center)
SKU/Catalog Number
A05895-1
Size
80 µl
Description
Boster Bio Anti-HIP2 Antibody (Center) (Catalog # A05895-1). Tested in IHC-P, IF, WB application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-HIP2 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05895-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This HIP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-139 amino acids from the Central region of human HIP2.
Reactive Species
A05895-1 is reactive to UBE2K in Human, Mouse
Calculated molecular weight
22.4 kDa
Background of UBE2K
HIP2 belongs to the ubiquitin-conjugating enzyme family. It binds selectively to a large region at the N terminus of huntingtin. This interaction is not influenced by the length of the huntingtin polyglutamine tract. This protein has been implicated in the degradation of huntingtin and suppression of apoptosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05895-1 is guaranteed for IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:10-1:50
WB: 1:1000
IHC-P: 1:100
Validation Images & Assay Conditions
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Fluorescent image of Hela cell stained with HIP2 Antibody (Center).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HIP2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). HIP2 immunoreactivity is localized to Cytoplasm significantly.
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HIP2 Antibody (D124) western blot analysis in 293,mouse C2C12 cell line lysates (35ug/lane).This demonstrates the HIP2 antibody detected the HIP2 protein (arrow).
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Immunohistochemical analysis of paraffin-embedded H. small intestine section using HIP2 Antibody (Center). A05895-1 was diluted at 1:100 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Specific Publications For Anti-HIP2 Antibody (Center) (A05895-1)
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