Anti-Histone deacetylase 6 HDAC6 Antibody

Product Name

Anti-Histone deacetylase 6 HDAC6 Antibody

View all HDAC6 Antibodies

SKU/Catalog Number

A00595

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Histone deacetylase 6 HDAC6 Antibody catalog # A00595. Tested in WB,ICC/IF,IHC,IP applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Histone deacetylase 6 HDAC6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00595)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human CD71 around the phosphorylation site of S24.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00595 is reactive to HDAC6 in Human

Applications

A00595 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

131.419kDa

Background of HDAC6

Transcriptional regulator that binds to DNA as a dimer or as a tetramer, but not as a monomer. Binds to G-doublets in an A/T-rich environment; the preferred motif is a tandem repeat of 5'-. ATTGGTTA-3' combined with a 5'-TTATTA-3' box. Binds to nucleosomes By similarity. Binds to chromatin and interacts selectively with histone H3 that is not methylated at 'Lys-4', not phosphorylated at 'Thr-3' and not methylated at 'Arg-2'. Functions as a sensor of histone H3 modifications that are important for the epigenetic regulation of gene expression. Functions as a transcriptional activator and promotes the expression of otherwise tissue-specific self-antigens in the thymus, which is important for self tolerance and the avoidance of autoimmune reactions.

Nagamine K., Nat. Genet. 17:393-398(1997).
Aaltonen J., Nat. Genet. 17:399-403(1997).
Hattori M., Nature 405:311-319(2000).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1,000-1:5,000
ICC: 1:50-1:200
IHC: 1:50-1:200
FC: 1:50-1:100

Validation Images & Assay Conditions

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Figure 2. Immunocytochemistry staining of HDAC6 using Anti-Histone deacetylase 6 HDAC6 Antibody (A00595).

ICC staining HDAC6 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde

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Figure 1. Western blotting validation for Anti-Histone deacetylase 6 HDAC6 Antibody A00595

Western blot analysis of HDAC6 on Jurkat cells lysates using anti-HDAC6 antibody at 1/1

Gene/Protein Information For HDAC6 (Source: Uniprot.org, NCBI)

Alternative Names

EC 3.5.1.98; HD6FLJ16239; histone deacetylase 6; JM21; KIAA0901 HDAC6 CPBHM, HD6, JM21, PPP1R90 histone deacetylase 6 histone deacetylase 6|protein phosphatase 1, regulatory subunit 90|tubulin-lysine deacetylase HDAC6

For more info on HDAC6, check out the HDAC6 Infographic

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In this infographic, you will see the following information for HDAC6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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