Click image to see more details
Product Info Summary
| SKU: | M00595 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-HDAC6/Histone Deacetylase 6 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00595
BM4725 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-HDAC6/Histone Deacetylase 6 Rabbit Monoclonal Antibody catalog # M00595. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HDAC6/Histone Deacetylase 6 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00595)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
HEE-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human HDAC6
Reactive Species
M00595 is reactive to HDAC6 in Human
Observed Molecular Weight
160 kDa
Calculated molecular weight
131.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00595 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-5000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
Positive Control
WB: human Hela whole cell, human K562 whole cell, human Jurkat whole cell, human HepG2 whole cell, human SIHA whole cell, human A431 whole cell
Validation Images & Assay Conditions
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Western blot analysis of HDAC6 using anti-HDAC6 antibody (M00595).
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: human SIHA whole cell lysates,
Lane 6: human A431 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC6 antigen affinity purified monoclonal antibody (M00595) at 1: 5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HDAC6 at approximately 160 kDa. The expected band size for HDAC6 is at 131 kDa.
Specific Publications For Anti-HDAC6/Histone Deacetylase 6 Rabbit Monoclonal Antibody (M00595)
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Customer Q&As
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16 Customer Q&As for Anti-HDAC6/Histone Deacetylase 6 Rabbit Monoclonal Antibody
Question
Is this M00595 anti-HDAC6/Histone Deacetylase 6 Rabbit Monoclonal antibody reactive to the isotypes of HDAC6?
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
The immunogen of M00595 anti-HDAC6/Histone Deacetylase 6 Rabbit Monoclonal antibody is A synthesized peptide derived from human HDAC6 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-05-04
Question
Does M00595 anti-HDAC6/Histone Deacetylase 6 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
It shows on the product datasheet, M00595 anti-HDAC6/Histone Deacetylase 6 Rabbit Monoclonal antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-27
Question
Is a blocking peptide available for product anti-HDAC6/Histone Deacetylase 6 Rabbit Monoclonal antibody (M00595)?
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
We do provide the blocking peptide for product anti-HDAC6/Histone Deacetylase 6 Rabbit Monoclonal antibody (M00595). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-03-06
Question
We are currently using anti-HDAC6/Histone Deacetylase 6 Rabbit Monoclonal antibody M00595 for human tissue, and we are satisfied with the IP results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
The anti-HDAC6/Histone Deacetylase 6 Rabbit Monoclonal antibody (M00595) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-20
Question
I see that the anti-HDAC6/Histone Deacetylase 6 Rabbit Monoclonal antibody M00595 works with IP, what is the protocol used to produce the result images on the product page?
K. Lewis
Verified customer
Asked: 2020-01-27
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-01-27
Question
We are interested in using your anti-HDAC6/Histone Deacetylase 6 Rabbit Monoclonal antibody for positive regulation of epithelial cell migration studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
Thank you for your inquiry. This M00595 anti-HDAC6/Histone Deacetylase 6 Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for IP, IF, IHC, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-01-15
Question
We have observed staining in human adenohypophysis. Any tips? Is anti-HDAC6/Histone Deacetylase 6 Rabbit Monoclonal antibody supposed to stain adenohypophysis positively?
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
From what I have seen in literature adenohypophysis does express HDAC6. From what I have seen in Uniprot.org, HDAC6 is expressed in adenohypophysis, brain, ovary placenta, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have HDAC6 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 10048485, 15772651
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Ovary, and Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-12-11
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-HDAC6/Histone Deacetylase 6 Rabbit Monoclonal antibody M00595. Let me know if you need anything else.
B. Moore
Verified customer
Asked: 2019-10-29
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-29
Question
See below the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-HDAC6/Histone Deacetylase 6 Rabbit Monoclonal antibody M00595. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-12
Question
Does anti-HDAC6/Histone Deacetylase 6 Rabbit Monoclonal antibody M00595 work for IP with cervix carcinoma erythroleukemia?
G. Kulkarni
Verified customer
Asked: 2018-09-03
Answer
According to the expression profile of cervix carcinoma erythroleukemia, HDAC6 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-HDAC6/Histone Deacetylase 6 Rabbit Monoclonal antibody M00595 will work for IP with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2018-09-03
Question
Is there a BSA free version of anti-HDAC6/Histone Deacetylase 6 Rabbit Monoclonal antibody M00595 available?
C. Thomas
Verified customer
Asked: 2018-03-27
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-HDAC6/Histone Deacetylase 6 Rabbit Monoclonal antibody M00595 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-03-27
Question
My question regards to test anti-HDAC6/Histone Deacetylase 6 Rabbit Monoclonal antibody M00595 on human cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-HDAC6/Histone Deacetylase 6 Rabbit Monoclonal antibody M00595 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
S. Wu
Verified customer
Asked: 2017-11-09
Answer
The products we sell, including anti-HDAC6/Histone Deacetylase 6 Rabbit Monoclonal antibody M00595, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-11-09
Question
Our lab used your anti-HDAC6/Histone Deacetylase 6 Rabbit Monoclonal antibody for IHC on ovary placenta in a previous project. I am using human, and We want to use the antibody for ICC next. We are interested in examining ovary placenta as well as adenohypophysis in our next experiment. Could you please give me some suggestion on which antibody would work the best for ICC?
Z. Bhatt
Verified customer
Asked: 2017-09-22
Answer
I looked at the website and datasheets of our anti-HDAC6/Histone Deacetylase 6 Rabbit Monoclonal antibody and it appears that M00595 has been validated on human in both IHC and ICC. Thus M00595 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-09-22
Question
I have a question about product M00595, anti-HDAC6/Histone Deacetylase 6 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-06-27
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00595 anti-HDAC6/Histone Deacetylase 6 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-06-27
Question
I was wanting to use your anti-HDAC6/Histone Deacetylase 6 Rabbit Monoclonal antibody for IP for human cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma erythroleukemia identification?
R. Brown
Verified customer
Asked: 2017-06-14
Answer
You can see on the product datasheet, M00595 anti-HDAC6/Histone Deacetylase 6 Rabbit Monoclonal antibody has been validated for IP, IF, IHC, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-06-14
Question
My colleagues were happy with the WB result of your anti-HDAC6/Histone Deacetylase 6 Rabbit Monoclonal antibody. However we have seen positive staining in adenohypophysis nucleus. cytoplasm. perikaryon using this antibody. Is that expected? Could you tell me where is HDAC6 supposed to be expressed?
G. Gonzalez
Verified customer
Asked: 2014-08-27
Answer
From literature, adenohypophysis does express HDAC6. Generally HDAC6 expresses in nucleus. cytoplasm. perikaryon. Regarding which tissues have HDAC6 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 10048485, 15772651
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Ovary, and Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2014-08-27
