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Product Info Summary
| SKU: | A06717 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Histone H1 HIST1H1B Antibody
SKU/Catalog Number
A06717
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Histone H1 HIST1H1B Antibody catalog # A06717. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Histone H1 HIST1H1B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06717)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human Histone H1. AA range:1-50
Reactive Species
A06717 is reactive to HIST1H1B in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
22.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06717 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Recommend Dilution
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
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Immunofluorescence analysis of HUVEC cells, using Histone H1 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western Blot analysis of 3T3 cells using Histone H1 Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
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Western blot analysis of lysates from COLO cells, using Histone H1 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using Histone H1 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunofluorescence analysis of Hela cell. 1,Histone H1 Polyclonal Antibody (red) was diluted at 1:200 (4° overnight). α-SMA Monoclonal Antibody (6A12) (green) was diluted at 1:200 (4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).
Specific Publications For Anti-Histone H1 HIST1H1B Antibody (A06717)
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-Histone H1 HIST1H1B Antibody
Question
Is this A06717 anti-Histone H1 antibody reactive to the isotypes of HIST1H1B?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
The immunogen of A06717 anti-Histone H1 antibody is Synthesized peptide derived from human Histone H1 around the non-phosphorylation site of T17. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-27
Question
We are currently using anti-Histone H1 antibody A06717 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-05-03
Answer
The anti-Histone H1 antibody (A06717) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-03
Question
I was wanting to use your anti-Histone H1 antibody for WB for mouse ovarian carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse ovarian carcinoma identification?
Verified Customer
Verified customer
Asked: 2017-06-14
Answer
You can see on the product datasheet, A06717 anti-Histone H1 antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse ovarian carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-06-14
