Anti-Histone H2A.X (E145) H2AFX Antibody

H2AX antibody

Boster Bio Anti-Histone H2A.X (E145) H2AFX Antibody catalog # A00241-2. Tested in IHC applications. This antibody reacts with Human,Mouse,Rat.

Product Info Summary

SKU: A00241-2
Size: 100ul
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC

Customers Who Bought This Also Bought

Product Name

Anti-Histone H2A.X (E145) H2AFX Antibody

View all H2AX Antibodies

SKU/Catalog Number

A00241-2

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Histone H2A.X (E145) H2AFX Antibody catalog # A00241-2. Tested in IHC applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Histone H2A.X (E145) H2AFX Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00241-2)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human FADD around the phosphorylation site of S194.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00241-2 is reactive to H2AX in Human, Mouse, Rat

Applications

A00241-2 is guaranteed for IHC Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

15.145kDa

Background of H2AX

Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. In cooperation with TAX1BP1 promotes disassembly of E2-E3 ubiquitin protein ligase complexes in IL-1R and TNFR-1 pathways; affected are at least E3 ligases TRAF6, TRAF2 and BIRC2, and E2 ubiquitin-conjugating enzymes UBE2N and UBE2D3. In cooperation with TAX1BP1 promotes ubiquitination of UBE2N and proteasomal degradation of UBE2N and UBE2D3. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Deubiquitinates TRAF6 probably acting on 'Lys-63'-linked polyubiquitin. Upon T-cell receptor (TCR)-mediated T-cell activation, deubiquitinates 'Lys-63'-polyubiquitin chains on MALT1 thereby mediating disassociation of the CBM (CARD11:BCL10:MALT1) and IKK complexes and preventing sustained IKK activation. Deubiquitinates NEMO/IKBKG; the function is facilitated by TNIP1 and leads to inhibition of NF-kappa-B activation. Upon stimulation by bacterial peptidoglycans, probably deubiquitinates RIPK2. Can also inhibit I-kappa-B-kinase (IKK) through a non-catalytic mechanism which involves polyubiquitin; polyubiquitin promotes association with IKBKG and prevents IKK MAP3K7-mediated phosphorylation. Targets TRAF2 for lysosomal degradation. In vitro able to deubiquitinate 'Lys-11'-, 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system. Required for LPS-induced production of proinflammatory cytokines and IFN beta in LPS-tolerized macrophages.

Opipari A.W. Jr., J. Biol. Chem. 265:14705-14708(1990).
Mungall A.J., Nature 425:805-811(2003).
Eliopoulos A.G., J. Virol. 73:1023-1035(1999).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IHC: 1:50-1:200

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

Gene/Protein Information For H2AX (Source: Uniprot.org, NCBI)

Gene Name

H2AX

Full Name

Histone H2AX

Weight

15.145kDa

Superfamily

histone H2A family

Alternative Names

H2A histone family, member X; H2A.X; H2a/x; H2AX histone; H2AXhistone H2A.x; YH2AX H2AX H2A.X, H2A/X, H2AFX H2A.X variant histone histone H2AX|H2A histone family member X|H2AX histone|histone H2A.x

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on H2AX, check out the H2AX Infographic

H2AX infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for H2AX: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for A00241-2

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-Histone H2A.X (E145) H2AFX Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-Histone H2A.X (E145) H2AFX Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

0 Customer Q&As for Anti-Histone H2A.X (E145) H2AFX Antibody

Order DetailsPrice
A00241-2-100ul

100ul

$405

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
Eddy test
In stock
Order Product
A00241-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$405.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.