Anti-Histone H2A.Z H2afz Rabbit Monoclonal Antibody

H2AZ1 antibody

Boster Bio Anti-Histone H2A.Z H2afz Rabbit Monoclonal Antibody catalog # M16777. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M16777
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC, ICC, WB

Product Name

Anti-Histone H2A.Z H2afz Rabbit Monoclonal Antibody

View all H2AZ1 Antibodies

SKU/Catalog Number

M16777

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Histone H2A.Z H2afz Rabbit Monoclonal Antibody catalog # M16777. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Histone H2A.Z H2afz Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M16777)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

EOO-8

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Histone H2A.Z

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M16777 is reactive to H2AZ1 in Human, Mouse, Rat

Applications

M16777 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

37 kDa

Calculated molecular weight

13.553kDa

Background of H2AZ1

Putative transcription factor involved in pancreas development and function.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200

Validation Images & Assay Conditions

Gene/Protein Information For H2AZ1 (Source: Uniprot.org, NCBI)

Gene Name

H2AZ1

Full Name

Histone H2A.Z

Weight

13.553kDa

Superfamily

histone H2A family

Alternative Names

Histone H2A.Z H2AZ1 H2A.Z-1, H2A.z, H2A/z, H2AFZ, H2AZ H2A.Z variant histone 1 histone H2A.Z|H2A histone family member Z|H2AZ histone

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on H2AZ1, check out the H2AZ1 Infographic

H2AZ1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for H2AZ1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-Histone H2A.Z H2afz Rabbit Monoclonal Antibody

Question

Is there a BSA free version of anti-Histone H2A.Z Rabbit Monoclonal antibody M16777 available?

Verified Customer

Verified customer

Asked: 2019-11-15

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Histone H2A.Z Rabbit Monoclonal antibody M16777 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-11-15

Question

We are currently using anti-Histone H2A.Z Rabbit Monoclonal antibody M16777 for rat tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-20

Answer

The anti-Histone H2A.Z Rabbit Monoclonal antibody (M16777) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-20

Question

My question regarding product M16777, anti-Histone H2A.Z Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-06-04

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M16777 anti-Histone H2A.Z Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-06-04

Question

Would anti-Histone H2A.Z Rabbit Monoclonal antibody M16777 work on horse IHC with brain?

Verified Customer

Verified customer

Asked: 2019-01-24

Answer

Our lab technicians have not tested anti-Histone H2A.Z Rabbit Monoclonal antibody M16777 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Histone H2A.Z Rabbit Monoclonal antibody M16777 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse brain in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-01-24

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-Histone H2A.Z Rabbit Monoclonal antibody M16777. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-06-25

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-06-25

Order DetailsPrice
M16777

100μL

$370
M16777-30ul

30μL sample

$99
M16777-carrier-free

Carrier Free

$370

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M16777
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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