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- Table of Contents
HIST1H3A antibody (M12477-10) performs well in IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 6 Publications
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Immunofluorescent analysis of Hela cells, using Histone H3 Antibody.
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Anti-Histone H3 HIST1H3A Rabbit Monoclonal Antibody
HIST1H3A antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC, ICC, WB |
Tested Applications
HIST1H3A antibody was positively detected by IHC in:
Human tonsil tissue, Human colon cancer tissue, Rat thymus tissue, Mouse spleen tissue, Human esophageal carcinoma tissue, mouse brain tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
HIST1H3A antibody was positively detected by ICC/IF in:
Hela cell
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Recommend
Tested
Recommend
Rat
Recommend
Tested
Recommend
Mouse
Recommend
Tested
Recommend
Product Information
Product Name
Anti-Histone H3 HIST1H3A Rabbit Monoclonal Antibody
View all HIST1H3A Antibodies
View all HIST1H3A Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
HDE-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Histone H3
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
17 kDa
Calculated Molecular Weight
15.4 kDa
Cite This Product
Anti-Histone H3 HIST1H3A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M12477-10)
Protocols
Immunohistochemistry (IHC) protocol for HIST1H3A antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for HIST1H3A antibody
Publications
Application:
Species:
Mechanistic insights into miR-584-5p-mediated Inhibition of PDLSCs osteogenic differentiation through H2AFZ upregulation and RUNX2 suppression
Published: 2025-10-07
Journal: CELLULAR AND MOLECULAR LIFE SCIENCES
Impact Factor: 6.20
PMID: 41055656
Nanographene Oxide Promotes Angiogenesis by Regulating Osteoclast Differentiation and Platelet-Derived Growth Factor Secretion
Published: 2024-08-06
Journal: ACS Nano
Application: WB
Impact Factor: 15.80
Species: Mouse
PMID: 39105734
Exploring the Anti-Inflammatory Effect of Tryptanthrin by Regulating TLR4/MyD88/ROS/NF-κB, JAK/STAT3, and Keap1/Nrf2 Signaling Pathways
Published: 2024-07-03
Journal: ACS Omega
Application: WB
Impact Factor: 3.70
Species: Mouse
PMID: 39035974
Oxidized DJ-1 activates the p-IKK/NF-κB/Beclin1 pathway by binding PTEN to induce autophagy and exacerbate myocardial ischemia-reperfusion injury
Published: 2024-03-18
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: WB
Impact Factor: 4.20
Species: Rat
PMID: 38508437
Sox9 mediates autophagy-dependent vascular smooth muscle cell phenotypic modulation and transplant arteriosclerosis
Published: 2022-09-21
Journal: iScience
Application: WB
Impact Factor: 6.11
Species: Rat
PMID: 36204267
PI3Kγ promotes vascular smooth muscle cell phenotypic modulation and transplant arteriosclerosis via a SOX9-dependent mechanism
Published: 2018-09-18
Journal: EBioMedicine
Application: WB
Impact Factor: 6.18
Species: Human,Rat
PMID: 30241919
Review
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Product Promise
We promise Anti-Histone H3 HIST1H3A Rabbit Monoclonal Antibody performs excellently in relevant applications for IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
M12477-10
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 28, 2026