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Product Info Summary
| SKU: | A03856-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-Hjurp Antibody Picoband®
SKU/Catalog Number
A03856-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Hjurp Antibody Picoband® catalog # A03856-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Hjurp Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03856-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Hjurp recombinant protein (Position: Q13-T574).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03856-1 is reactive to Hjurp in Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
84 kDa
Calculated molecular weight
127459 MW
Background of HJURP
Holliday junction recognition protein is a protein in humans that is encoded by the HJURP gene. HJURP (holliday junction recognition protein) is a protein coding gene. Diseases associated with HJURP include fibrillary astrocytoma. Among its related pathways are DNA damage and chromosome maintenance. Centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Prevents CENPA-H4 tetramerization and prevents premature DNA binding by the CENPA-H4 tetramer. Directly binds holliday junctions.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03856-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Mouse
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: mouse stomach tissue, mouse liver tissue, mouse kidney tissue, mouse RAW2647 whole cell, rat stomach tissue, rat liver tissue, rat kidney tissue
FCM: ANA-1 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Hjurp using anti-Hjurp antibody (A03856-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse stomach tissue lysates,
Lane 2: mouse liver tissue lysates,
Lane 3: mouse kidney tissue lysates,
Lane 4: mouse RAW264.7 whole cell lysates,
Lane 5: rat stomach tissue lysates,
Lane 6: rat liver tissue lysates,
Lane 7: rat kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hjurp antigen affinity purified polyclonal antibody (Catalog # A03856-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hjurp at approximately 84 kDa. The expected band size for Hjurp is at 84 kDa.
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Figure 2. Flow Cytometry analysis of ANA-1 cells using anti-Hjurp antibody (A03856-1).
Overlay histogram showing ANA-1 cells stained with A03856-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Hjurp Antibody (A03856-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For Hjurp (Source: Uniprot.org, NCBI)
Gene Name
Hjurp
Full Name
Holliday junction recognition protein
Weight
127459 MW
Alternative Names
AF4/FMR2 family member 4;ALL1-fused gene from chromosome 5q31 protein;Protein AF-5q31;Major CDK9 elongation factor-associated protein;AFF4;AF5Q31, MCEF;HSPC092; HJURP FAKTS, URLC9, hFLEG1 Holliday junction recognition protein Holliday junction recognition protein|14-3-3-associated AKT substrate|fetal liver expressing gene 1|fetal liver-expressing gene 1 protein|up-regulated in lung cancer 9
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Hjurp, check out the Hjurp Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Hjurp: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hjurp Antibody Picoband® (A03856-1)
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