Click image to see more details
Product Info Summary
| SKU: | A30721 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-HLA-DOA/Hla Doa Antibody
SKU/Catalog Number
A30721
Size
100ul
Form
Liquid
Description
Boster Bio Anti-HLA-DOA/Hla Doa Antibody (Catalog# A30721). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HLA-DOA/Hla Doa Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30721)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human HLA-DOA. Immunogen sequence location: 71-120
Reactive Species
A30721 is reactive to HLA-DOA in Human
Observed Molecular Weight
72 kDa
Calculated molecular weight
27.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30721 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:40000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Western Blot analysis of various cells using HLA-DOα Polyclonal Antibody
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Western blot analysis of lysates from COLO cells, using HLA-DOA Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-HLA-DOA/Hla Doa Antibody (A30721)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-HLA-DOA/Hla Doa Antibody
Question
Can you help my question with product A30721, anti-HLA-DOA/Hla Doa antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
N. Jones
Verified customer
Asked: 2020-03-11
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30721 anti-HLA-DOA/Hla Doa antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-11
Question
Does anti-HLA-DOA/Hla Doa antibody A30721 work for WB with amniotic fluid?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
According to the expression profile of amniotic fluid, HLA-DOA is highly expressed in amniotic fluid. So, it is likely that anti-HLA-DOA/Hla Doa antibody A30721 will work for WB with amniotic fluid.
Boster Scientific Support
Answered: 2020-03-11
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for amniotic fluid using anti-HLA-DOA/Hla Doa antibody A30721. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-11
Question
My lab would like to test anti-HLA-DOA/Hla Doa antibody A30721 on human amniotic fluid for research purposes, then I may be interested in using anti-HLA-DOA/Hla Doa antibody A30721 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
N. Jha
Verified customer
Asked: 2019-08-28
Answer
The products we sell, including anti-HLA-DOA/Hla Doa antibody A30721, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-28
Question
We are currently using anti-HLA-DOA/Hla Doa antibody A30721 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-10-04
Answer
The anti-HLA-DOA/Hla Doa antibody (A30721) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-04
Question
Is a blocking peptide available for product anti-HLA-DOA/Hla Doa antibody (A30721)?
B. Kulkarni
Verified customer
Asked: 2018-08-06
Answer
We do provide the blocking peptide for product anti-HLA-DOA/Hla Doa antibody (A30721). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-08-06
