Product Info Summary
SKU: | M01235-2 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human |
Host: | Mouse |
Application: | ELISA, Flow Cytometry, IP, IHC-F, ICC |
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Product info
Product Name
Anti-HLA-G Purified Azide Free Monoclonal Antibody
SKU/Catalog Number
M01235-2
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-HLA-G Purified Azide Free Monoclonal Antibody (Catalog# M01235-2). Tested in Flow Cytometry, IP, IHC-F, ICC, ELISA application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-HLA-G Purified Azide Free Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01235-2)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, azide-free
Clonality
Monoclonal
Clone Number
MEM-G/9
Isotype
Mouse IgG1
Immunogen
Recombinant human HLA-G refolded with beta2-microglobulin and peptide. The antibody MEM-G/9 reacts with an extracellular epitope on native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. Reactivity with HLA-G3 was also reported. The antibody MEM-G/9 is standard reagent thoroughly validated during 3rd International Conference on HLA-G (Paris, 2003).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01235-2 is reactive to HLA-G in Human
Applications
M01235-2 is guaranteed for ELISA, Flow Cytometry, IP, IHC-F, ICC Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
38.224kDa
Background of HLA G
Human leukocyte antigen G (HLA-G), belonging to MHC class I glycoproteins, plays important roles in both physiological and pathological immunotolerance. It gives an inhibitory signal to cytotoxic T cells, NK cells, monocytes, and some other immune cells. It also induces regulatory T cells and anti-inflammatory macrophages. HLA-G is important e.g. for maternal tolerance to the fetus, and for immunomodulation in particular adult tissues, such as in cornea, pancreatic islets, thymus and other. On the other hand, it is expressed in many solid and hematologic malignancies, where it contributes to evasion of the immune surveillance. HLA-G expression pattern in cancer is an important prognostic factor regarding a poor clinical outcome. Unlike most other MHC glycoproteins, HLA-G acts as an immune checkpoint molecule rather than as an antigen presenting molecule. It concerns both transmembrane and soluble HLA-G isoforms. Among other, HLA-G can promote Th2 immunological response and downregulate Th1 immunological response. For its benefits regarding allograft tolerance, including embryo implantation, soluble HLA-G (sHLA-G) can be used as a marker of developmental potential of embryos during the process of in vitro fertilization. Similarly, sHLA-G concentrations in maternal serum are decreased in preeclampsia. Transplanted patients with increased sHLA-G serum levels have improved allograft acceptance. On the other hand, increased sHLA-G can also indicate presence of malignant (sometimes also of benign) tumor cells. Another important topic is induction of HLA-G expression (sometimes associated with shedding of HLA-G from the cell surface) by some anti-cancer or anti-viral therapies, which can weaken the therapy effect. Monitoring of HLA-G in patients thus has a wide usage.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 0.3-4 μg/ml; positive control: JEG-3 human choriocarcinoma cell line.
Immunocytochemistry: 2-5 μg/ml.
ELISA: The antibody MEM-G/9 has been tested as the capture antibody in a sandwich ELISA for analysis of human HLA-G in combination with antibody B2M-01 or with antibody W6/32. Coating antibody 10 μg/ml, detection antibody (biotin or peroxidase conjugate) 1 μg/ml.
Immunohistochemistry: 1-4 μg/ml.
Validation Images & Assay Conditions
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Protein Target Info & Infographic
Gene/Protein Information For HLA-G (Source: Uniprot.org, NCBI)
Gene Name
HLA-G
Full Name
HLA class I histocompatibility antigen, alpha chain G
Weight
38.224kDa
Superfamily
MHC class I family
Alternative Names
b2 microglobulin; HLA class I histocompatibility antigen, alpha chain G; HLA class I molecule; HLA G antigen; HLA G; HLA-6.0; HLA-G histocompatibility antigen, class I, G; HLAG; HLA-G; major histocompatibility complex, class I, G; MHC Class I Antigen G; MHC-G HLA-G MHC-G major histocompatibility complex, class I, G HLA class I histocompatibility antigen, alpha chain G|HLA G antigen|HLA-G histocompatibility antigen, class I, G|MHC class I antigen G|MHC class Ib antigen|b2 microglobulin|mutant MHC class I antigen|mutant MHC class Ib antigen
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HLA-G, check out the HLA-G Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HLA-G: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HLA-G Purified Azide Free Monoclonal Antibody (M01235-2)
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No publications found for M01235-2
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