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Product Info Summary
| SKU: | A30494 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-HLX/Hlx11 Antibody
SKU/Catalog Number
A30494
Size
100ul
Form
Liquid
Description
Boster Bio Anti-HLX/Hlx11 Antibody (Catalog# A30494). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HLX/Hlx11 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30494)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human HLX1. Immunogen sequence location: 321-370
Reactive Species
A30494 is reactive to HLX in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
50.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30494 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:10000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Western Blot analysis of various cells using HLX1 Polyclonal Antibody diluted at 1:1000
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Immunofluorescence analysis of MCF7 cells, using HLX1 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from COLO205 cells, using HLX1 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HeLa cells using HLX1 antibody.
Specific Publications For Anti-HLX/Hlx11 Antibody (A30494)
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1 Customer Q&As for Anti-HLX/Hlx11 Antibody
Question
We are currently using anti-HLX/Hlx11 antibody A30494 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
The anti-HLX/Hlx11 antibody (A30494) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-13
