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- Table of Contents
HMGB1 antibody (M00066-1) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 9 Publications
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IHC analysis of HMGB1 using anti-HMGB1 antibody (M00066-1).
HMGB1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-HMGB1 Antibody (M00066-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
HMGB1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-HMGB1 Antibody (M00066-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-HMGB1/Hmg 1 Rabbit Monoclonal Antibody
HMGB1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
HMGB1 antibody was positively detected by WB in:
human Hela whole cell, human 293T whole cell, human K562 whole cell, human Jurkat whole cell, human HeLa- WT whole cell, human HeLa-HMGB1 KO whole cell, rat brain tissue, rat liver tissue, mouse brain tissue, mouse liver tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
HMGB1 antibody was positively detected by IHC in:
human colorectal adenocarcinoma tissue, human lung adenocarcinoma tissue, human spleen tissue, human prostatic acinar adenocarcinoma tissue, human cervical squamous carcinoma tissue, Rat liver tissue, Rat kidney tissue, Mouse skeletal muscle - gastrocnemius tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
HMGB1 antibody was positively detected by ICC/IF in:
Hela cell
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:30
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
FC
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Product Information
Product Name
Anti-HMGB1/Hmg 1 Rabbit Monoclonal Antibody
View all HMGB1 Antibodies
View all HMGB1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AAC-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human HMGB1
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
25 kDa
Calculated Molecular Weight
24.9 kDa
Cite This Product
Anti-HMGB1/Hmg 1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00066-1)
Protocols
Western Blot protocol for HMGB1 antibody
Immunohistochemistry (IHC) protocol for HMGB1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for HMGB1 antibody
Publications
Application:
Species:
Biomimetic-Nanoparticle-Enhanced Photothermal Immunotherapy: Targeted Delivery of Near-Infrared Region II Agents and Immunoadjuvants for Tumor Immunogenicity
Published: 2025-03-04
Journal: Biomaterials Research
Application: IF,FC
Impact Factor: 9.60
Species: Mouse
PMID: 40040955
n-3 polyunsaturated fatty acids alleviate the progression of obesity-related osteoarthritis and protect cartilage through inhibiting the HMGB1-RAGE/TLR4 signaling pathway
Published: 2024-01-12
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: ELISA
Impact Factor: 4.80
Species: Mouse
PMID: 38218011
Abdominal paracentesis drainage attenuates intestinal inflammation in rats with severe acute pancreatitis by inhibiting the HMGB1-mediated TLR4 signaling pathway
Published: 2021-03-07
Journal: WORLD JOURNAL OF GASTROENTEROLOGY
Application: IHC
Impact Factor: 5.74
Species: Rat
PMID: 33727772
Abdominal paracentesis drainage ameliorates myocardial injury in severe experimental pancreatitis rats through suppressing oxidative stress
Published: 2020-01-07
Journal: WORLD JOURNAL OF GASTROENTEROLOGY
Application: ELISA
Impact Factor: 3.67
Species: Rat
PMID: 31933513
H19 promote calcium oxalate nephrocalcinosis-induced renal tubular epithelial cell injury via a ceRNA pathway
Published: 2019-11-14
Journal: EBioMedicine
Application: WB,IHC
Impact Factor: 6.68
Species: Mouse
PMID: 31735555
High-Mobility Group Box 1 Protein Regulates Autophagy in LO2 Cells Following Anoxia-Reoxygenation Injury
Published: 2018-06-12
Journal: TRANSPLANTATION PROCEEDINGS
Application: WB
Impact Factor: 0.81
Species: Human
PMID: 29880383
Propofol inhibits the release of interleukin-6, 8 and tumor necrosis factor-α correlating with high-mobility group box 1 expression in lipopolysaccharides-stimulated RAW 264.7 cells
Published: 2017-10-26
Journal: BMC Anesthesiology
Application: WB
Impact Factor: 1.53
Species: Mouse
PMID: 29073894
The HMGB1‑IL‑17A axis contributes to hypoxia/reoxygenation injury via regulation of cardiomyocyte apoptosis and autophagy
Published: 2017-10-20
Journal: Molecular Medicine Reports
Application: WB
Impact Factor: 1.69
Species: Rat
PMID: 29115425
MicroRNA-205‑5b inhibits HMGB1 expression in LPS-induced sepsis
Published: 2016-05-27
Journal: INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
Application: WB
Impact Factor: 2.35
Species: Mouse
PMID: 27246725
Review
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Product Promise
We promise Anti-HMGB1/Hmg 1 Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
M00066-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 15, 2026