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HNF4A antibody (M00389-2) performs well in IHC, WB and shows reactivity with Human, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of HNF-4-alpha using anti-HNF-4-alpha antibody (M00389-2).
HNF-4-alpha was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-HNF-4-alpha Antibody (M00389-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
HNF-4-alpha was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-HNF-4-alpha Antibody (M00389-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
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Anti-HNF-4-alpha Antibody Picoband® (monoclonal, 6C8E9)
HNF4A antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Rat | Mouse | IHC, WB |
Tested Applications
HNF4A antibody was positively detected by WB in:
human HepG2 whole cell, rat liver tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
HNF4A antibody was positively detected by IHC in:
rat colon tissue, rat liver tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Rat
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Mouse
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-HNF-4-alpha Antibody Picoband® (monoclonal, 6C8E9)
View all HNF4A Antibodies
View all HNF4A Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
6C8E9
Isotype
Mouse IgG2a
Immunogen
E.coli-derived human HNF-4-alpha recombinant protein (Position: Q164-I474). Human HNF-4-alpha shares 95% and 96% amino acid (aa) sequence identity with mouse and rat HNF-4-alpha, respectively.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
53 kDa
Calculated Molecular Weight
52.8 kDa
Background of HNF4A
Hepatocyte nuclear factor 4 alpha (HNF4A), also known as NR2A1, is a nuclear receptor that in humans is encoded by the HNF4A gene. It is mapped to 20q13.12. HNF4A is a nuclear transcription factor that binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene plays a role in development of the liver, kidney, and intestines. HNF4A is required for the PXR and CAR-mediated transcriptional activation of CYP3A4. This gene also plays a pivotal role in the expression and synthesis of SHBG, an important glycoprotein made primarily in the liver, which in addition to lowering insulin-resistance also serves in reducing levels of free Oestrogen as-well as prolonging the half-life of Testosterone.
Cite This Product
Anti-HNF-4-alpha Antibody Picoband® (monoclonal, 6C8E9) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00389-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
99%
High Homology
Dog
98%
High Homology
Cow
97%
High Homology
Rat
96%
High Homology
Mouse
95%
High Homology
Sheep
94%
High Homology
Rabbit
89%
Moderate Homology
Zebrafish
77%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for HNF4A antibody
Immunohistochemistry (IHC) protocol for HNF4A antibody
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Product Promise
We promise Anti-HNF-4-alpha Antibody Picoband® (monoclonal, 6C8E9) performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M00389-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 4, 2026