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Product Info Summary
| SKU: | A03691-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-hnRNP U/p120/HNRNPU Antibody Picoband™
SKU/Catalog Number
A03691-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-hnRNP U/p120/HNRNPU Antibody Picoband™ catalog # A03691-3. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-hnRNP U/p120/HNRNPU Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03691-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human hnRNP U/p120/HNRNPU recombinant protein (Position: E262-L563).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03691-3 is reactive to HNRNPU in Human, Mouse, Rat
Applications
A03691-3 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
120 kDa
Calculated molecular weight
90.584kDa
Background of hnRNP U
Heterogeneous nuclear ribonucleoprotein U is a protein that in humans is encoded by the HNRNPU gene. This gene encodes a member of a family of proteins that bind nucleic acids and function in the formation of ribonucleoprotein complexes in the nucleus with heterogeneous nuclear RNA (hnRNA). The encoded protein has affinity for both RNA and DNA, and binds scaffold-attached region (SAR) DNA. Mutations in this gene have been associated with epileptic encephalopathy, early infantile, 54. A pseudogene of this gene has been identified on chromosome 14.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunofluorescence, 5 μg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3 μg/1x106 cells, Human, Rat
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of hnRNP U/p120/HNRNPU using anti-hnRNP U/p120/HNRNPU antibody (A03691-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MOLT-4 whole cell lysates,
Lane 2: human Daudi whole cell lysates,
Lane 3: human HEL whole cell lysates,
Lane 4: human U251 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-hnRNP U/p120/HNRNPU antigen affinity purified polyclonal antibody (Catalog # A03691-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for hnRNP U/p120/HNRNPU at approximately 120 kDa. The expected band size for hnRNP U/p120/HNRNPU is at 90 kDa.
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Figure 2. IHC analysis of hnRNP U/p120/HNRNPU using anti-hnRNP U/p120/HNRNPU antibody (A03691-3).
hnRNP U/p120/HNRNPU was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-hnRNP U/p120/HNRNPU Antibody (A03691-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of hnRNP U/p120/HNRNPU using anti-hnRNP U/p120/HNRNPU antibody (A03691-3).
hnRNP U/p120/HNRNPU was detected in a paraffin-embedded section of human gastric carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-hnRNP U/p120/HNRNPU Antibody (A03691-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of hnRNP U/p120/HNRNPU using anti-hnRNP U/p120/HNRNPU antibody (A03691-3).
hnRNP U/p120/HNRNPU was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-hnRNP U/p120/HNRNPU Antibody (A03691-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of hnRNP U/p120/HNRNPU using anti-hnRNP U/p120/HNRNPU antibody (A03691-3).
hnRNP U/p120/HNRNPU was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-hnRNP U/p120/HNRNPU Antibody (A03691-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 6. IHC analysis of hnRNP U/p120/HNRNPU using anti-hnRNP U/p120/HNRNPU antibody (A03691-3).
hnRNP U/p120/HNRNPU was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-hnRNP U/p120/HNRNPU Antibody (A03691-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 7. IHC analysis of hnRNP U/p120/HNRNPU using anti-hnRNP U/p120/HNRNPU antibody (A03691-3).
hnRNP U/p120/HNRNPU was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-hnRNP U/p120/HNRNPU Antibody (A03691-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 8. IHC analysis of hnRNP U/p120/HNRNPU using anti-hnRNP U/p120/HNRNPU antibody (A03691-3).
hnRNP U/p120/HNRNPU was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-hnRNP U/p120/HNRNPU Antibody (A03691-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 9. IHC analysis of hnRNP U/p120/HNRNPU using anti-hnRNP U/p120/HNRNPU antibody (A03691-3).
hnRNP U/p120/HNRNPU was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-hnRNP U/p120/HNRNPU Antibody (A03691-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 10. IHC analysis of hnRNP U/p120/HNRNPU using anti-hnRNP U/p120/HNRNPU antibody (A03691-3).
hnRNP U/p120/HNRNPU was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-hnRNP U/p120/HNRNPU Antibody (A03691-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 11. IF analysis of hnRNP U/p120/HNRNPU using anti-hnRNP U/p120/HNRNPU antibody (A03691-3).
hnRNP U/p120/HNRNPU was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-hnRNP U/p120/HNRNPU Antibody (A03691-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 12. Flow Cytometry analysis of HEL cells using anti-hnRNP U/p120/HNRNPU antibody (A03691-3).
Overlay histogram showing HEL cells stained with A03691-3 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-hnRNP U/p120/HNRNPU Antibody (A03691-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Figure 13. Flow Cytometry analysis of C6 cells using anti-hnRNP U/p120/HNRNPU antibody (A03691-3).
Overlay histogram showing C6 cells stained with A03691-3 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-hnRNP U/p120/HNRNPU Antibody (A03691-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 14. IF analysis of hnRNP U/p120/HNRNPU using anti-hnRNP U/p120/HNRNPU antibody (A03691-3).
hnRNP U/p120/HNRNPU was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-hnRNP U/p120/HNRNPU Antibody (A03691-3) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 15. IF analysis of hnRNP U/p120/HNRNPU using anti-hnRNP U/p120/HNRNPU antibody (A03691-3).
hnRNP U/p120/HNRNPU was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-hnRNP U/p120/HNRNPU Antibody (A03691-3) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 16. IF analysis of hnRNP U/p120/HNRNPU using anti-hnRNP U/p120/HNRNPU antibody (A03691-3).
hnRNP U/p120/HNRNPU was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-hnRNP U/p120/HNRNPU Antibody (A03691-3) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For HNRNPU (Source: Uniprot.org, NCBI)
Gene Name
HNRNPU
Full Name
Heterogeneous nuclear ribonucleoprotein U
Weight
90.584kDa
Alternative Names
heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A); heterogeneous nuclear ribonucleoprotein U; hnRNP U; HNRPU; p120 nuclear protein; p120; pp120; SAFA; SAF-AhnRNPU; Scaffold attachment factor A; U21.1 HNRNPU DEE54, EIEE54, GRIP120-AS1, HNRPU, SAF-A, SAFA, U21.1, hnRNP U, pp120, HNRNPU heterogeneous nuclear ribonucleoprotein U heterogeneous nuclear ribonucleoprotein U|HNRNPU antisense RNA 1|heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A)|nuclear p120 ribonucleoprotein|p120 nuclear protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HNRNPU, check out the HNRNPU Infographic
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In this infographic, you will see the following information for HNRNPU: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-hnRNP U/p120/HNRNPU Antibody Picoband™ (A03691-3)
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