Product Info Summary
SKU: | M02006 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Mouse |
Application: | ELISA, Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-Homeobox protein OTX2 OTX2 Monoclonal Antibody
SKU/Catalog Number
M02006
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Homeobox protein OTX2 OTX2 Monoclonal Antibody catalog # M02006. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Homeobox protein OTX2 OTX2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02006)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
1H12C4B5
Isotype
IgG
Immunogen
Purified recombinant fragment of human OTX2 expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02006 is reactive to OTX2 in Human
Applications
M02006 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
31.636kDa
Background of OTX2
Indoleamine 2, 3-dioxygenase1 (IDO1) is a 41-42 kD intracellular enzyme that catabolizes tryptophan into kynurenine. IDO1 modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. IDO1 effects on immune suppression are due to decreased tryptophan availability and the generation of tryptophan metabolites, resulting in negative effects on T lymphocytes, including proliferation, function and survival. IDO1 may be involved in the suppression of the immune response to tumors, and blocking the IDO1 pathway may be a potential target for immuno and cancer therapy. IDO1 is expressed in a wide variety of tissues and can be upregulated by interferon gamma and other inflammatory cytokines.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Figure 5. ELISA validation of OTX2 using Anti-Homeobox protein OTX2 OTX2 Monoclonal Antibody (M02006).
ELISA analysis of OTX2 antibody.Click image to see more details
Figure 4. Flow Cytometry validation of OTX2 using Anti-Homeobox protein OTX2 OTX2 Monoclonal Antibody (M02006).
Flow cytometric (FCM) analysis of HepG2 cells using OTX2 Monoclonal Antibody (green) and negative control (purple).Click image to see more details
Figure 3. Immunofluorescent staining data of OTX2 using Anti-Homeobox protein OTX2 OTX2 Monoclonal Antibody (M02006).
Immunofluorescence (IF) analysis of HepG2 cells using OTX2 Monoclonal Antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Click image to see more details
Figure 2. Immunohistochemistry validation of OTX2 using Anti-Homeobox protein OTX2 OTX2 Monoclonal Antibody (M02006).
Immunohistochemistry (IHC) analysis of paraffin-embedded prostate tissues (left) and colon cancer tissues (right) with DAB staining using OTX2 Monoclonal Antibody.For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-Homeobox protein OTX2 OTX2 Monoclonal Antibody M02006
Western Blot (WB) analysis using OTX2 Monoclonal Antibody against HepG2 (1)Protein Target Info & Infographic
Gene/Protein Information For OTX2 (Source: Uniprot.org, NCBI)
Gene Name
OTX2
Full Name
Homeobox protein OTX2
Weight
31.636kDa
Superfamily
paired homeobox family
Alternative Names
homeobox protein OTX2; MCOPS5; MGC45000; orthodenticle homeobox 2; orthodenticle homolog 2 (Drosophila); Orthodenticle homolog 2; Otx2 OTX2 CPHD6, MCOPS5 orthodenticle homeobox 2 homeobox protein OTX2|orthodenticle homolog 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on OTX2, check out the OTX2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for OTX2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Homeobox protein OTX2 OTX2 Monoclonal Antibody (M02006)
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No publications found for M02006
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Customer Reviews
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