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Product Info Summary
| SKU: | A05019 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Homeodomain-only protein HOPX Antibody
SKU/Catalog Number
A05019
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Homeodomain-only protein HOPX Antibody (Catalog # A05019). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
HOPX antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Homeodomain-only protein HOPX Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05019)
Host
Rabbit
Contents
HOPX antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
HOPX antibody was raised against a 16 amino acid peptide near the amino terminus of human HOPX. The immunogen is located within the first 50 amino acids of HOPX.
Cross-reactivity
HOPX antibody is human specific. At least three isoforms of HOPX are known to exist; this antibody will detect all three isoforms.
Reactive Species
A05019 is reactive to HOPX in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
8.3 kDa
Background of HOPX
HOPX is a small homeodomain protein that lacks normally conserved residues required for DNA binding (1). It is thought to act downstream of NKX2-5 and modulates serum response factor (SRF)-dependent cardiac-specific gene expression and cardiac development (1). HOPX also acts a tumor suppressor gene in multiple tumors and cancer cell lines (2,3). HOPX has been reported to regulate the proliferation/differentiation homeostasis in different cell types, including keratinocytes (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05019 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
HOPX antibody can be used for detection of HOPX by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of HOPX in human brain tissue lysate with HOPX antibody at (A) 1 and (B) 2 μg/ml.
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Immunohistochemistry of HOPX in human heart tissue with HOPX antibody at 5 μg/mL.
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Immunofluorescence of HOPX in human heart tissue with HOPX antibody at 20 μg/mL.
Specific Publications For Anti-Homeodomain-only protein HOPX Antibody (A05019)
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