Anti-HOOK2 Antibody Picoband™

HOOK2 antibody

Boster Bio Anti-HOOK2 Antibody Picoband™ catalog # A08854-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A08854-1
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-HOOK2 Antibody Picoband™

View all HOOK2 Antibodies

SKU/Catalog Number

A08854-1

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-HOOK2 Antibody Picoband™ catalog # A08854-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HOOK2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08854-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human HOOK2 recombinant protein (Position: E500-R702).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A08854-1 is reactive to HOOK2 in Human, Mouse, Rat

Applications

A08854-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Background of HOOK2

Protein Hook homolog 2 (HK2) is a protein that in humans is encoded by the HOOK2 gene. This gene is mapped to 19p13.13. Hook proteins are cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules, and more divergent C-terminal domains, which mediate binding to organelles. The Drosophila Hook protein is a component of the endocytic compartment.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 0.5-1μg/ml
Flow Cytometry, 1-3μg/1x106 cells
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For HOOK2 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q96ED9

Gene ID

29911

Gene Name

HOOK2

Full Name

Protein Hook homolog 2

Weight

83.207kDa

Superfamily

hook family

Alternative Names

FLJ26218; hHK2; HK2h-hook2; hook homolog 2 (Drosophila); protein Hook homolog 2 HOOK2 HK2 hook microtubule tethering protein 2 protein Hook homolog 2|h-hook2|hHK2|hook homolog 2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on HOOK2, check out the HOOK2 Infographic

HOOK2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for HOOK2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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7 Customer Q&As for Anti-HOOK2 Antibody Picoband™

Question

Will A08854-1 anti-HOOK2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-01-10

Answer

You can see on the product datasheet, A08854-1 anti-HOOK2 antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-01-10

Question

I was wanting to use your anti-HOOK2 antibody for ELISA for mouse adenohypophysis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse adenohypophysis identification?

Verified Customer

Verified customer

Asked: 2019-11-07

Answer

As indicated on the product datasheet, A08854-1 anti-HOOK2 antibody has been validated for ELISA, Flow Cytometry, IHC-P, IHC-F, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse adenohypophysis in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-11-07

Question

Is a blocking peptide available for product anti-HOOK2 antibody (A08854-1)?

Verified Customer

Verified customer

Asked: 2019-07-11

Answer

We do provide the blocking peptide for product anti-HOOK2 antibody (A08854-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-07-11

Question

My question regarding product A08854-1, anti-HOOK2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

S. Carter

Verified customer

Asked: 2018-06-22

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A08854-1 anti-HOOK2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-06-22

Question

We are currently using anti-HOOK2 antibody A08854-1 for human tissue, and we are content with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2017-07-18

Answer

The anti-HOOK2 antibody (A08854-1) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-07-18

Question

Here is the WB image, lot number and protocol we used for adenohypophysis using anti-HOOK2 antibody A08854-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2017-07-12

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-07-12

Question

Is this A08854-1 anti-HOOK2 antibody reactive to the isotypes of HOOK2?

G. Carter

Verified customer

Asked: 2016-08-23

Answer

The immunogen of A08854-1 anti-HOOK2 antibody is E.coli-derived human HOOK2 recombinant protein (Position: E500-R702). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2016-08-23

Conjugation

how to order through PO

Size

Total: $315

Size:100μg

SKU:A08854-1

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A08854-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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