Click image to see more details
Product Info Summary
| SKU: | M01142-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IHC, WB |
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Product info
Product Name
Anti-HP1 gamma Rabbit Monoclonal Antibody
SKU/Catalog Number
M01142-1
BM5304 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-HP1 gamma Rabbit Monoclonal Antibody catalog # M01142-1. Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HP1 gamma Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01142-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACGB-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human HP1 gamma
Reactive Species
M01142-1 is reactive to CBX3 in Human, Mouse, Rat
Observed Molecular Weight
21 kDa
Calculated molecular weight
20.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01142-1 is guaranteed for Flow Cytometry, IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
IP 1:20
FC 1:20
Positive Control
WB: human Hela whole cell, human K562 whole cell, human THP-1 whole cell, human U-937 whole cell, rat lung tissue, rat PC-12 whole cell, mouse lung tissue, mouse NIH/3T3 whole cell
Validation Images & Assay Conditions
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Western blot analysis of HP1 Gamma using anti-HP1 Gamma antibody (M01142-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human THP-1 whole cell lysates,
Lane 4: human U-937 whole cell lysates,
Lane 5: rat lung tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse lung tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HP1 Gamma antigen affinity purified monoclonal antibody (Catalog # M01142-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HP1 Gamma at approximately 21 kDa. The expected band size for HP1 Gamma is at 21 kDa.
Specific Publications For Anti-HP1 gamma Rabbit Monoclonal Antibody (M01142-1)
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8 Customer Q&As for Anti-HP1 gamma Rabbit Monoclonal Antibody
Question
I see that the anti-HP1 gamma Monoclonal antibody M01142-1 works with IP, what is the protocol used to produce the result images on the product page?
S. Walker
Verified customer
Asked: 2020-03-12
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-03-12
Question
Will M01142-1 anti-HP1 gamma Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-10
Answer
As indicated on the product datasheet, M01142-1 anti-HP1 gamma Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-10
Question
What is the sample target on Western Blot analysis in your product image for M01142-1?
Verified customer
Asked: 2019-12-09
Answer
Western Blot analysis of the Anti-HP1 Gamma Rabbit Monoclonal Antibody (M01142-1) expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate.
Boster Scientific Support
Answered: 2019-12-10
Question
I was wanting to use your anti-HP1 gamma Monoclonal antibody for IP for human epithelial cell of pancreas on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human epithelial cell of pancreas identification?
Verified Customer
Verified customer
Asked: 2019-10-02
Answer
You can see on the product datasheet, M01142-1 anti-HP1 gamma Monoclonal antibody has been tested for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human epithelial cell of pancreas in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-02
Question
Is this M01142-1 anti-HP1 gamma Monoclonal antibody reactive to the isotypes of CBX3?
Verified Customer
Verified customer
Asked: 2018-10-03
Answer
The immunogen of M01142-1 anti-HP1 gamma Monoclonal antibody is A synthesized peptide derived from human HP1 gamma Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR) . Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-10-03
Question
Here is the WB image, lot number and protocol we used for epithelial cell of pancreas using anti-HP1 gamma Monoclonal antibody M01142-1. Please let me know if you require anything else.
N. Thomas
Verified customer
Asked: 2017-01-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-01-04
Question
Is a blocking peptide available for product anti-HP1 gamma Monoclonal antibody (M01142-1)?
O. Lewis
Verified customer
Asked: 2016-04-12
Answer
We do provide the blocking peptide for product anti-HP1 gamma Monoclonal antibody (M01142-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2016-04-12
Question
Would anti-HP1 gamma Monoclonal antibody M01142-1 work for IP with epithelial cell of pancreas?
J. Johnson
Verified customer
Asked: 2016-03-10
Answer
According to the expression profile of epithelial cell of pancreas, CBX3 is highly expressed in epithelial cell of pancreas. So, it is likely that anti-HP1 gamma Monoclonal antibody M01142-1 will work for IP with epithelial cell of pancreas.
Boster Scientific Support
Answered: 2016-03-10
