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Product Info Summary
| SKU: | M00668 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-HPRT HPRT1 Monoclonal Antibody
SKU/Catalog Number
M00668
BM5361 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-HPRT HPRT1 Monoclonal Antibody catalog # M00668. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HPRT HPRT1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00668)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADAB-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human HPRT
Reactive Species
M00668 is reactive to HPRT1 in Human, Mouse, Rat
Observed Molecular Weight
25 kDa
Calculated molecular weight
24.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00668 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
IP 1:20
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2W dilution for 1 hour at room temperature.
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Western blot analysis of HPRT1 using anti-HPRT1 antibody (M00668).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549- WT whole cell lysates,
Lane 2: human A549-HPRT1 KO whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HPRT1 antigen affinity purified monoclonal antibody (M00668) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HPRT1 at approximately 25 kDa. The expected band size for HPRT1 is at 25 kDa.
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All lanes use the Antibody at 1:2W dilution for 1 hour at room temperature.
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Western blot analysis of HPRT expression in A549 cell lysate.
Specific Publications For Anti-HPRT HPRT1 Monoclonal Antibody (M00668)
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