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Product Info Summary
| SKU: | PB9220 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-HRG Antibody Picoband™
SKU/Catalog Number
PB9220
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HRG Antibody Picoband™ catalog # PB9220. Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HRG Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9220)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human HRG recombinant protein (Position: V19-V200). Human HRG shares 71% and 69% amino acid (aa) sequences identity with mouse and rat HRG, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9220 is reactive to HRG in Human
Applications
PB9220 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
60 kDa
Calculated molecular weight
59.578kDa
Background of HPRG
Histidine-rich glycoprotein (HRG) is a protein that in humans is encoded by the HRG gene. It is mapped to 3q27.3. HRG contains two cystatin-like domains and is located in plasma and platelets. The protein can bind heme, dyes and divalent metal ions. It also can inhibit rosette formation and interacts with heparin, thrombospondin and plasminogen. Two of the protein's effects, the inhibition of fibrinolysis and the reduction of inhibition of coagulation, indicate a potential prothrombotic effect. Mutations in this gene lead to thrombophilia due to abnormal histidine-rich glycoprotein levels. HRG is thought to have multiple roles as a protein in the human blood, including roles in immunity, angiogenesis and coagulation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
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Anti-HRG antibody, PB9220, Western blotting
All lanes: Anti HRG (PB9220) at 0.5ug/ml
WB: Recombinant Human HRG Protein 0.5ng
Predicted bind size: 40KD
Observed bind size: 40KD
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Anti-HRG antibody, PB9220, Western blotting
All lanes: Anti HRG (PB9220) at 0.5ug/ml
WB: Human Placenta Tissue Lysate at 50ug
Predicted bind size: 60KD
Observed bind size: 60KD
Click image to see more details
Anti-HRG antibody, PB9220, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For HRG (Source: Uniprot.org, NCBI)
Gene Name
HRG
Full Name
Histidine-rich glycoprotein
Weight
59.578kDa
Alternative Names
DKFZp779H1622; Histidine-proline-rich glycoprotein; histidine-rich glycoprotein; HPRG; HPRGHRGP; HRG HRG HPRGP, THPH11, HRG histidine rich glycoprotein histidine-rich glycoprotein|histidine-proline-rich glycoprotein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HRG, check out the HRG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HRG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HRG Antibody Picoband™ (PB9220)
Hello CJ!
No publications found for PB9220
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-HRG Antibody Picoband™
Question
We are currently using anti-HRG antibody PB9220 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
The anti-HRG antibody (PB9220) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-22
