Anti-HRI EIF2AK1 Antibody

EIF2AK1 antibody

Boster Bio Anti-HRI EIF2AK1 Antibody catalog # A05465. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse.

Product Info Summary

SKU: A05465
Size: 100μl
Reactive Species: Human, Monkey, Mouse
Host: Rabbit
Application: WB

Product Name

Anti-HRI EIF2AK1 Antibody

View all EIF2AK1 Antibodies

SKU/Catalog Number

A05465

Size

100μl

Form

Liquid

Description

Boster Bio Anti-HRI EIF2AK1 Antibody catalog # A05465. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HRI EIF2AK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05465)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Isotype

IgG

Immunogen

Synthesized peptide derived from the C-terminal region of human HRI. at AA rangle: 540-620

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A05465 is reactive to EIF2AK1 in Human, Monkey, Mouse

Applications

A05465 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For EIF2AK1 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9BQI3

Gene ID

27102

Gene Name

EIF2AK1

Full Name

Eukaryotic translation initiation factor 2-alpha kinase 1

Weight

71.106kDa

Superfamily

protein kinase superfamily

Alternative Names

EC 2.7.11; eukaryotic translation initiation factor 2-alpha kinase 1; HCR; heme sensitive initiation factor 2a kinase; Heme-controlled repressor; Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase; Heme-regulated inhibitor; heme-regulated initiation factor 2-alpha kinase; heme-regulated repressor; Hemin-sensitive initiation factor 2-alpha kinase; HRIEC 2.7.11.1; KIAA1369heme regulated initiation factor 2 alpha kinase EIF2AK1 HCR, HRI, LEMSPAD, hHRI eukaryotic translation initiation factor 2 alpha kinase 1 eukaryotic translation initiation factor 2-alpha kinase 1|heme regulated initiation factor 2 alpha kinase|heme sensitive initiation factor 2a kinase|heme-controlled repressor|heme-regulated eukaryotic initiation factor eIF-2-alpha kinase|heme-regulated inhibitor|heme-regulated repressor|hemin-sensitive initiation factor 2-alpha kinase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on EIF2AK1, check out the EIF2AK1 Infographic

EIF2AK1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for EIF2AK1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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3 Customer Q&As for Anti-HRI EIF2AK1 Antibody

Question

Would anti-HRI antibody A05465 work on canine WB with placenta tongue?

Verified Customer

Verified customer

Asked: 2020-05-07

Answer

Our lab technicians have not tested anti-HRI antibody A05465 on canine. You can run a BLAST between canine and the immunogen sequence of anti-HRI antibody A05465 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine placenta tongue in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-05-07

Question

My question regarding product A05465, anti-HRI antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

O. Huang

Verified customer

Asked: 2019-09-17

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05465 anti-HRI antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-09-17

Question

We are currently using anti-HRI antibody A05465 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, monkey, mouse. Is it true that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2019-06-06

Answer

The anti-HRI antibody (A05465) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-06-06

Size

Total: $399

SKU:A05465

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
A05465
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$399.00

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