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Product Info Summary
| SKU: | A05465 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Monkey, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-HRI EIF2AK1 Antibody
SKU/Catalog Number
A05465
Size
100μl
Form
Liquid
Description
Boster Bio Anti-HRI EIF2AK1 Antibody catalog # A05465. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Monkey.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HRI EIF2AK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05465)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human EIF2AK1. AA range:571-620
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A05465 is reactive to EIF2AK1 in Human, Monkey, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
71.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05465 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC: 1:100-300
ELISA 1:20000
IF 1:100-300
Validation Images & Assay Conditions
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Western Blot analysis of various cells using HRI Polyclonal Antibody
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Western blot analysis of lysates from A549 and COS7 cells, using EIF2AK1 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from COLO205 cells using EIF2AK1 antibody.
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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
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Immunofluorescence analysis of A549. 1, primary Antibody (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000 (room temperature, 50min).3, Picture B: DAPI (blue) 10min.
Specific Publications For Anti-HRI EIF2AK1 Antibody (A05465)
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Customer Q&As
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3 Customer Q&As for Anti-HRI EIF2AK1 Antibody
Question
Would anti-HRI antibody A05465 work on canine WB with placenta tongue?
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
Our lab technicians have not tested anti-HRI antibody A05465 on canine. You can run a BLAST between canine and the immunogen sequence of anti-HRI antibody A05465 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine placenta tongue in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-05-07
Question
My question regarding product A05465, anti-HRI antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
O. Huang
Verified customer
Asked: 2019-09-17
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05465 anti-HRI antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-17
Question
We are currently using anti-HRI antibody A05465 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, monkey, mouse. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
The anti-HRI antibody (A05465) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-06
