Anti-Hydroxysteroid (17-beta) Dehydrogenase 4/HSD17B4 Antibody Picoband™

Product Name

Anti-Hydroxysteroid (17-beta) Dehydrogenase 4/HSD17B4 Antibody Picoband™

View all HSD17B4 Antibodies

SKU/Catalog Number

A02923

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Hydroxysteroid (17-beta) Dehydrogenase 4/HSD17B4 Antibody Picoband™ catalog # A02923. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Hydroxysteroid (17-beta) Dehydrogenase 4/HSD17B4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02923)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human HSD17B4 recombinant protein (Position: D510-L736). Human HSD17B4 shares 87.7% and 89% amino acid (aa) sequence identity with mouse and rat HSD17B4, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A02923 is reactive to HSD17B4 in Human, Mouse, Rat

Applications

A02923 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

80 kDa

Calculated molecular weight

79.686kDa

Background of HSD17B4

Peroxisomal multifunctional enzyme type 2 is a protein that in humans is encoded by the HSD17B4 gene. The protein encoded by this gene is a bifunctional enzyme that is involved in the peroxisomal beta-oxidation pathway for fatty acids. It also acts as a catalyst for the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids. Defects in this gene that affect the peroxisomal fatty acid beta-oxidation activity are a cause of D-bifunctional protein deficiency (DBPD). An apparent pseudogene of this gene is present on chromosome 8. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of HSD17B4 using anti-HSD17B4 antibody (A02923).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: rat liver tissue lysates,
Lane 3: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD17B4 antigen affinity purified polyclonal antibody (Catalog # A02923) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 fo r 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD17B4 at approximately 80 kDa. The expected band size for HSD17B4 is at 80 kDa.

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Figure 2. IHC analysis of HSD17B4 using anti-HSD17B4 antibody (A02923).
HSD17B4 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HSD17B4 Antibody (A02923) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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Figure 3. IHC analysis of HSD17B4 using anti-HSD17B4 antibody (A02923).
HSD17B4 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HSD17B4 Antibody (A02923) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Click image to see more details

Figure 4. IHC analysis of HSD17B4 using anti-HSD17B4 antibody (A02923).
HSD17B4 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HSD17B4 Antibody (A02923) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Gene/Protein Information For HSD17B4 (Source: Uniprot.org, NCBI)

Alternative Names

12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase; 7-alpha; D-bifunctional protein; EDH17B4; hydroxysteroid (17-beta) dehydrogenase 4; MPF-2; peroxisomal multifunctional enzyme type 2,17-beta-HSD IV; peroxisomal multifunctional protein 2,17beta-estradiol dehydrogenase type IV; peroxisomal; short chain dehydrogenase/reductase family 8C, member 1,3-alpha HSD17B4 DBP, MFE-2, MFP-2, MPF-2, PRLTS1, SDR8C1 hydroxysteroid 17-beta dehydrogenase 4 peroxisomal multifunctional enzyme type 2|17-beta-HSD 4|17-beta-HSD IV|17-beta-hydroxysteroid dehydrogenase 4|17beta-estradiol dehydrogenase type IV|3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase|D-3-hydroxyacyl-CoA dehydratase|D-bifunctional protein, peroxisomal|beta-hydroxyacyl dehydrogenase|beta-keto-reductase|epididymis secretory sperm binding protein|hydroxysteroid dehydrogenase 4|multifunctional protein 2|peroxisomal multifunctional protein 2|short chain dehydrogenase/reductase family 8C member 1

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