Anti-HSD17B6 Antibody

Product Name

Anti-HSD17B6 Antibody

View all HSD17B6 Antibodies

SKU/Catalog Number

PA1386

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-HSD17B6 Antibody catalog # PA1386. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HSD17B6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1386)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human HSD17B6.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1386 is reactive to HSD17B6 in Human

Applications

PA1386 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

35 kDa

Calculated molecular weight

35.966kDa

Background of HSD17B6

Hydroxysteroid 17-beta dehydrogenase 6 is an enzyme that in humans is encoded by the HSD17B6 gene. The protein encoded by this gene has both oxidoreductase and epimerase activities and is involved in androgen catabolism. Baker ME et al point out expression of 17beta-HSDs had an important role in the early evolution of the physiological response to androgens and estrogens. Biswas and Russell concluded that 17beta-HSD6 and RoDH play opposing roles in androgen action; 17beta-HSD6 inactivates 3alpha-adiol by conversion to androsterone and RoDH activates 3alpha-adiol by conversion to dihydrotestosterone. The synthesis of an active steroid hormone by back conversion of an inactive metabolite represents a potentially important mechanism by which the steady state level of a transcriptional effector can be regulated.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Anti-HSD17B6 antibody, PA1386, Western blotting
All lanes: Anti HSD17B6 (PA1386) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 35KD
Observed bind size: 35KD

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Figure 2. IHC analysis of HSD17B6 using anti-HSD17B6 antibody (PA1386).
HSD17B6 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HSD17B6 Antibody (PA1386) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Gene/Protein Information For HSD17B6 (Source: Uniprot.org, NCBI)

Alternative Names

17-beta-HSD 6; 17-beta-HSD6; 17-beta-hydroxysteroid dehydrogenase type 6; 3(alpha->beta)-hydroxysteroid epimerase; 3-alpha->beta-HSE; EC 1.1.1; EC 1.1.1.105; EC 1.1.1.62; EC 1.1.1.63; HSE3-hydroxysteroid epimerase; hydroxysteroid (17-beta) dehydrogenase 6 homolog (mouse); hydroxysteroid (17-beta) dehydrogenase 6; NAD+ -dependent 3 alpha-hydroxysteroid dehydrogenase 3-hydroxysteroid epimerase; Oxidative 3-alpha hydroxysteroid dehydrogenase; oxidative 3-alpha-hydroxysteroid-dehydrogenase; oxidoreductase; retinol dehydrogenase; RODH3(alpha->beta)-hydroxysteroid epimerasel; SDR9C6; short HSD17B6 HSE, RODH, SDR9C6 hydroxysteroid 17-beta dehydrogenase 6 17-beta-hydroxysteroid dehydrogenase type 6|17-beta-HSD 6|17-beta-hydroxysteroid dehydrogenase type 6 variant 1|17-beta-hydroxysteroid dehydrogenase type 6 variant 2|17-beta-hydroxysteroid dehydrogenase type 6 variant 3|3(alpha->beta)-hydroxysteroid epimerase|3(alpha->beta)-hydroxysteroid epimerasel|3-alpha->beta-HSE|3-hydroxysteroid epimerase|NAD+ -dependent 3 alpha-hydroxysteroid dehydrogenase 3-hydroxysteroid epimerase|hydroxysteroid (17-beta) dehydrogenase 6 homolog|oxidative 3-alpha-hydroxysteroid-dehydrogenase|oxidoreductase|retinol dehydrogenase|short chain dehydrogenase/reductase family 9C member 6

For more info on HSD17B6, check out the HSD17B6 Infographic

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