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- Table of Contents
HSD3B1 antibody (M02856) performs well in IF, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 4 Publications
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IF analysis of HSD3B1 using anti-HSD3B1 antibody (M02856).
HSD3B1 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-HSD3B1 Antibody (M02856) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
HSD3B1 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-HSD3B1 Antibody (M02856) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-HSD3B1 Antibody Picoband® (monoclonal, 3F3C/7F9C8)
HSD3B1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Mouse | IF, ICC, WB |
Tested Applications
HSD3B1 antibody was positively detected by WB in:
human placenta tissue, rat testis tissue, mouse testis tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
HSD3B1 antibody was positively detected by ICC/IF in:
U20S cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-HSD3B1 Antibody Picoband® (monoclonal, 3F3C/7F9C8)
View all HSD3B1 Antibodies
View all HSD3B1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
3F3C/7F9C8
Isotype
Mouse IgG2b
Immunogen
E.coli-derived human HSD3B1 recombinant protein (Position: Q17-Q373).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
42 kDa
Calculated Molecular Weight
42.3 kDa
Background of HSD3B1
HSD3B1 is a human gene that encodes for a 3beta-hydroxysteroid dehydrogenase/delta(5)-delta(4)isomerase type I or hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1. It is mapped to 1p12. The protein encoded by this gene is an enzyme that catalyzes the oxidative conversion of delta-5-3-beta-hydroxysteroid precursors into delta-4-ketosteroids, which leads to the production of all classes of steroid hormones. The encoded protein also catalyzes the interconversion of 3-beta-hydroxy- and 3-keto-5-alpha-androstane steroids.
Cite This Product
Anti-HSD3B1 Antibody Picoband® (monoclonal, 3F3C/7F9C8) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02856)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
94%
High Homology
Dog
80%
Moderate Homology
Cow
78%
Moderate Homology
Rabbit
78%
Moderate Homology
Sheep
78%
Moderate Homology
Rat
72%
Moderate Homology
Mouse
71%
Moderate Homology
Zebrafish
45%
Low homology
Drosophila
29%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for HSD3B1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for HSD3B1 antibody
Publications
Application:
Species:
Gut dysbiosis induces the development of asthenozoospermia through butanoate metabolism
Published: 2026-03-18
Journal: Frontiers in Immunology
Application: IF
Impact Factor: 5.90
Species: Mouse
PMID: 41929479
Autophagy-related 7 protein-dependent autophagy mediates resveratrol-caused upregulation of mitochondrial biogenesis and steroidogenesis in aged Leydig cell
Published: 2023-12-22
Journal: MOLECULAR BIOLOGY REPORTS
Application: WB,IHC
Impact Factor: 2.80
Species: Mouse
PMID: 38133746
Ameliorative Effect of VE, IGF-I, and hCG on the Fluoride-Induced Testosterone Release Suppression in Mice Leydig Cells
Published: 2017-05-01
Journal: BIOLOGICAL TRACE ELEMENT RESEARCH
Application: IF
Impact Factor: 2.40
Species: Mouse
PMID: 28462439
4-Methylcatechol inhibits cell growth and testosterone production in TM3 Leydig cells by reducing mitochondrial activity
Published: 2016-05-05
Journal: ANDROLOGIA
Application: WB
Impact Factor: 1.44
Species: Mouse
PMID: 27147129
Review
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Product Promise
We promise Anti-HSD3B1 Antibody Picoband® (monoclonal, 3F3C/7F9C8) performs excellently in relevant applications for IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M02856
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 6, 2026