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Product Info Summary
| SKU: | M02856 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-HSD3B1 Antibody Picoband® (monoclonal, 3F3C/7F9C8)
SKU/Catalog Number
M02856
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HSD3B1 Antibody Picoband® (monoclonal, 3F3C/7F9C8) catalog # M02856. Tested in IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-HSD3B1 Antibody Picoband® (monoclonal, 3F3C/7F9C8) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02856)
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
3F3C/7F9C8
Isotype
Mouse IgG2b
Immunogen
E.coli-derived human HSD3B1 recombinant protein (Position: Q17-Q373).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M02856 is reactive to HSD3B1 in Human, Mouse, Rat
Observed Molecular Weight
42 kDa
Calculated molecular weight
42.3 kDa
Background of HSD3B1
HSD3B1 is a human gene that encodes for a 3beta-hydroxysteroid dehydrogenase/delta(5)-delta(4)isomerase type I or hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1. It is mapped to 1p12. The protein encoded by this gene is an enzyme that catalyzes the oxidative conversion of delta-5-3-beta-hydroxysteroid precursors into delta-4-ketosteroids, which leads to the production of all classes of steroid hormones. The encoded protein also catalyzes the interconversion of 3-beta-hydroxy- and 3-keto-5-alpha-androstane steroids.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02856 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Positive Control
WB: human placenta tissue, rat testis tissue, mouse testis tissue
ICC/IF: U20S cell
Validation Images & Assay Conditions
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Western blot analysis of HSD3B1 using anti-HSD3B1 antibody (M02856).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: rat testis tissue lysates,
Lane 3: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HSD3B1 antigen affinity purified monoclonal antibody (Catalog # M02856) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for HSD3B1 at approximately 42 kDa. The expected band size for HSD3B1 is at 42 kDa.
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IF analysis of HSD3B1 using anti-HSD3B1 antibody (M02856).
HSD3B1 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-HSD3B1 Antibody (M02856) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Specific Publications For Anti-HSD3B1 Antibody Picoband® (monoclonal, 3F3C/7F9C8) (M02856)
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