Anti-HSF1 Monoclonal Antibody

Hsf1 antibody

Boster Bio Anti-HSF1 Monoclonal Antibody catalog # M00250. Tested in ELISA, IP, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: M00250
Size: 100ug
Reactive Species: Human, Mouse, Rat
Host: Rat
Application: ELISA, IP, IF, IHC, WB

Product Name

Anti-HSF1 Monoclonal Antibody

View all Hsf1 Antibodies

SKU/Catalog Number

M00250

Size

100ug

Form

liquid

Description

Boster Bio Anti-HSF1 Monoclonal Antibody catalog # M00250. Tested in ELISA, IP, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Cite This Product

Anti-HSF1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00250)

Host

Rat

Contents

Antibody concentration: 1 mg/ml, stored in PBS pH7.4, 50% glycerol, 0.09% sodium azide

Clonality

Monoclonal

Clone Number

10H8

Immunogen

Purified recombinant mouse HSF1 protein, with epitope mapping to amino acids 378-395

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00250 is reactive to Hsf1 in Human, Mouse, Rat

Applications

M00250 is guaranteed for ELISA, IP, IF, IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB (1:1000), IHC (1:1000), ICC/IF (1:200) ; optimal dilutions for assays should be determined by the user.

Validation Images & Assay Conditions

Gene/Protein Information For Hsf1 (Source: Uniprot.org, NCBI)

Uniprot ID

P38532

Gene ID

15499

Gene Name

Hsf1

Full Name

Heat shock factor protein 1

Weight

57.223kDa

Superfamily

HSF family

Alternative Names

heat shock factor protein 1; heat shock transcription factor 1HSTF1HSF 1; HSF1; HSTF 1; HSTF1 Hsf1|AA960185, HSTF, HSTF 1alpha, Hsf1beta, Hsf1|heat shock factor 1|heat shock factor protein 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Hsf1, check out the Hsf1 Infographic

Hsf1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Hsf1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

M00250 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Higher heat shock factor 1 expression in tumor stroma predicts poor prognosis in esophageal squamous cell carcinoma patients

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5 Customer Q&As for Anti-HSF1 Monoclonal Antibody

Question

My question regards using your anti-HSF1 Monoclonal antibody for r-mmu-3371453; regulation of hsf1-mediated heat shock response studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-02-05

Answer

Thank you for your inquiry. This M00250 anti-HSF1 Monoclonal antibody is tested on hela cells, mitotic hela cells. It is guaranteed to work for ELISA, IP, IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-02-05

Question

We are currently using anti-HSF1 Monoclonal antibody M00250 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-25

Answer

The anti-HSF1 Monoclonal antibody (M00250) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-25

Question

We bought anti-HSF1 Monoclonal antibody for IP on muscle a few months ago. I am using mouse, and We intend to use the antibody for IF next. We want examining muscle as well as leukemic t-cell in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?

Verified Customer

Verified customer

Asked: 2019-02-27

Answer

I looked at the website and datasheets of our anti-HSF1 Monoclonal antibody and I see that M00250 has been tested on mouse in both IP and IF. Thus M00250 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-02-27

Question

Our lab were satisfied with the WB result of your anti-HSF1 Monoclonal antibody. However we have seen positive staining in cervix carcinoma erythroleukemia nucleus using this antibody. Is that expected? Could you tell me where is HSF1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-05-01

Answer

From literature, cervix carcinoma erythroleukemia does express HSF1. Generally HSF1 expresses in nucleus. Regarding which tissues have HSF1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18220336, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Muscle, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2018-05-01

Question

We have been able to see staining in mouse testis. What should we do? Is anti-HSF1 Monoclonal antibody supposed to stain testis positively?

T. Taylor

Verified customer

Asked: 2014-07-24

Answer

From literature testis does express HSF1. From Uniprot.org, HSF1 is expressed in testis, muscle, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have HSF1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18220336, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Muscle, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2014-07-24

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M00250
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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