This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
HSPD1 antibody (M01280-3) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
Filter:
Flow Cytometry analysis of A431 cells using anti-HSPD1 antibody (M01280-3).
Overlay histogram showing A431 cells stained with M01280-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-HSPD1 Antibody (M01280-3,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing A431 cells stained with M01280-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-HSPD1 Antibody (M01280-3,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Bundle Save
Anti-Hsp60/HSPD1 Antibody Picoband® (monoclonal, 6G2)
HSPD1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Mouse | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
HSPD1 antibody was positively detected by WB in:
human Caco-2 whole cell, human A549 whole cell, human THP-1 whole cell, human SW620 whole cell, human U-937 whole cell, human HepG2 whole cell, rat RH35 whole cell, mouse RAW2467 whole cell,
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
HSPD1 antibody was positively detected by IHC in:
human intestinal cancer tissue, human lung cancer tissue, human mammary cancer tissue, mouse liver tissue, rat liver tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
HSPD1 antibody was positively detected by ICC/IF in:
A431 cell
HSPD1 antibody was positively detected by FC in:
A431 cell, HepG2 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Immunocytochemistry/Immunofluorescence
5μg/ml
Flow Cytometry (Fixed)
1-3μg/1x106 cells
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Rabbit
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Hsp60/HSPD1 Antibody Picoband® (monoclonal, 6G2)
View all HSPD1 Antibodies
View all HSPD1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
6G2
Isotype
Mouse IgG1
Immunogen
E.coli-derived human Hsp60/HSPD1 recombinant protein (Position: A260-Q496). Human Hsp60 shares 97% amino acid (aa) sequence identity with both mouse and rat Hsp60.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
60 kDa
Calculated Molecular Weight
61.1 kDa
Background of HSPD1
HSP60 is a member of the chaperonin class of protein factors, which include the Escherichia coli groEL protein and the Rubisco subunit-binding protein of chloroplasts. It acts as a costimulator of human regulatory CD4-positive/CD25 -positive T cells, which inhibit lymphoproliferation and IFNG and TNF secretion by CD4-positive and CD8-positive T cells. HSP60 enhances Treg activity via TLR2, leading to activation of an intracellular signaling cascade that included p38, as well as inhibition of ERK phosphorylation. Suppression of target T cells is mediated by both cell-to-cell contact and by secretion of TGFB and IL10, and it leads to downregulation of ERK, NFKB, and TBET expression. The self-molecule HSP60 can downregulate adaptive immune responses by upregulating Tregs through TLR2 signaling.
Cite This Product
Anti-Hsp60/HSPD1 Antibody Picoband® (monoclonal, 6G2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01280-3)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Rabbit
99%
High Homology
Dog
99%
High Homology
Monkey
98%
High Homology
Sheep
98%
High Homology
Cow
97%
High Homology
Mouse
97%
High Homology
Rat
97%
High Homology
Zebrafish
84%
Moderate Homology
Drosophila
69%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for HSPD1 antibody
Immunohistochemistry (IHC) protocol for HSPD1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for HSPD1 antibody
Flow Cytometry (FC) protocol for HSPD1 antibody
Publications
Application:
Species:
PRM-based quantitative proteomics analysis of altered HSP abundance in villi and decidua of patients with early missed abortion
Published: 2023-08-16
Journal: Proteome Science
Application: WB,IHC
Impact Factor: 2.00
Species: Human
PMID: 37587463
Review
Have you used Anti-Hsp60/HSPD1 Antibody Picoband® (monoclonal, 6G2)?
Submit a review and receive an Amazon gift card.
$30 for a review with an image
Submit A ReviewProduct Promise
We promise Anti-Hsp60/HSPD1 Antibody Picoband® (monoclonal, 6G2) performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M01280-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Sep 29, 2026