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Product Info Summary
| SKU: | M01103-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Hsp90 alpha HSP90AA1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01103-1
BM4194 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Hsp90 alpha HSP90AA1 Rabbit Monoclonal Antibody catalog # M01103-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Hsp90 alpha HSP90AA1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01103-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
CCF-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Hsp90 alpha
Reactive Species
M01103-1 is reactive to HSP90AA1 in Human, Mouse, Rat
Observed Molecular Weight
90 kDa
Calculated molecular weight
84.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01103-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: HeLa cell
IHC: human breast cancer tissue
ICC/IF: HeLa cell
Validation Images & Assay Conditions
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Western blot analysis on HeLa cell lysate using HSP90A Antibody.
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Immunohistochemical analysis of paraffin-embedded human breast cancer, using Hsp90 alpha antibody.
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Immunofluorescent analysis of HeLa cells, using Hsp90 alpha Antibody.
Specific Publications For Anti-Hsp90 alpha HSP90AA1 Rabbit Monoclonal Antibody (M01103-1)
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Hsp90 alpha HSP90AA1 Rabbit Monoclonal Antibody
Question
Does M01103-1 anti-Hsp90 alpha Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
J. Li
Verified customer
Asked: 2020-04-17
Answer
You can see on the product datasheet, M01103-1 anti-Hsp90 alpha Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-17
Question
My question regards using your anti-Hsp90 alpha Rabbit Monoclonal antibody for hsp90 chaperone cycle for steroid hormone receptors (shr) studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
Thank you for your inquiry. This M01103-1 anti-Hsp90 alpha Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-01-17
Question
I have attached the WB image, lot number and protocol we used for t-cell using anti-Hsp90 alpha Rabbit Monoclonal antibody M01103-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-30
Question
I see that the anti-Hsp90 alpha Rabbit Monoclonal antibody M01103-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-08-22
Question
We want to test anti-Hsp90 alpha Rabbit Monoclonal antibody M01103-1 on human t-cell for research purposes, then I may be interested in using anti-Hsp90 alpha Rabbit Monoclonal antibody M01103-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-05
Answer
The products we sell, including anti-Hsp90 alpha Rabbit Monoclonal antibody M01103-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-05
Question
We have tried in the past anti-Hsp90 alpha Rabbit Monoclonal antibody for IHC on middle temporal gyrus in a previous project. I am using mouse, and I plan to use the antibody for WB next. I am looking for examining middle temporal gyrus as well as melanoma in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
I have checked the website and datasheets of our anti-Hsp90 alpha Rabbit Monoclonal antibody and I see that M01103-1 has been validated on mouse in both IHC and WB. Thus M01103-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-07-04
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for t-cell using anti-Hsp90 alpha Rabbit Monoclonal antibody M01103-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-27
Question
Is this M01103-1 anti-Hsp90 alpha Rabbit Monoclonal antibody reactive to the isotypes of HSP90AA1?
Verified Customer
Verified customer
Asked: 2019-06-19
Answer
The immunogen of M01103-1 anti-Hsp90 alpha Rabbit Monoclonal antibody is A synthesized peptide derived from human Hsp90 alpha. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-19
Question
We are currently using anti-Hsp90 alpha Rabbit Monoclonal antibody M01103-1 for mouse tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-08-23
Answer
The anti-Hsp90 alpha Rabbit Monoclonal antibody (M01103-1) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-23
Question
Would anti-Hsp90 alpha Rabbit Monoclonal antibody M01103-1 work for WB with t-cell?
Verified Customer
Verified customer
Asked: 2018-07-17
Answer
According to the expression profile of t-cell, HSP90AA1 is highly expressed in t-cell. So, it is likely that anti-Hsp90 alpha Rabbit Monoclonal antibody M01103-1 will work for WB with t-cell.
Boster Scientific Support
Answered: 2018-07-17
Question
I was wanting to use your anti-Hsp90 alpha Rabbit Monoclonal antibody for WB for human t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human t-cell identification?
S. Krishna
Verified customer
Asked: 2018-02-01
Answer
It shows on the product datasheet, M01103-1 anti-Hsp90 alpha Rabbit Monoclonal antibody has been validated for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-02-01
Question
My colleagues were happy with the WB result of your anti-Hsp90 alpha Rabbit Monoclonal antibody. However we have observed positive staining in melanoma nucleus. using this antibody. Is that expected? Could you tell me where is HSP90AA1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-01-11
Answer
According to literature, melanoma does express HSP90AA1. Generally HSP90AA1 expresses in nucleus. Regarding which tissues have HSP90AA1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 2492519, 10409742
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 18318008, 24275569
Lymphoblast, Pubmed ID: 14654843
Melanoma, Pubmed ID: 17081065
Peripheral blood lymphocyte, Pubmed ID: 2780322
Pituitary, Pubmed ID: 16807684
Placenta, Pubmed ID: 2527334, 15489334
Placenta, and Teratocarcinoma, Pubmed ID: 14702039
Platelet, Pubmed ID: 18088087
Renal cell carcinoma, Pubmed ID: 10508479, 11274138
T-cell, Pubmed ID: 19367720
Boster Scientific Support
Answered: 2018-01-11
Question
Is there a BSA free version of anti-Hsp90 alpha Rabbit Monoclonal antibody M01103-1 available?
Verified Customer
Verified customer
Asked: 2018-01-03
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Hsp90 alpha Rabbit Monoclonal antibody M01103-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-01-03
Question
We have seen staining in human brain. Do you have any suggestions? Is anti-Hsp90 alpha Rabbit Monoclonal antibody supposed to stain brain positively?
C. Anderson
Verified customer
Asked: 2017-09-14
Answer
From what I have seen in literature brain does express HSP90AA1. From what I have seen in Uniprot.org, HSP90AA1 is expressed in middle temporal gyrus, peripheral blood lymphocyte, placenta, placenta teratocarcinoma, brain, cajal-retzius cell fetal brain cortex, heart, renal cell carcinoma, lymphoblast, cervix carcinoma, pituitary, t-cell, platelet, liver, leukemic t-cell, melanoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have HSP90AA1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 2492519, 10409742
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 18318008, 24275569
Lymphoblast, Pubmed ID: 14654843
Melanoma, Pubmed ID: 17081065
Peripheral blood lymphocyte, Pubmed ID: 2780322
Pituitary, Pubmed ID: 16807684
Placenta, Pubmed ID: 2527334, 15489334
Placenta, and Teratocarcinoma, Pubmed ID: 14702039
Platelet, Pubmed ID: 18088087
Renal cell carcinoma, Pubmed ID: 10508479, 11274138
T-cell, Pubmed ID: 19367720
Boster Scientific Support
Answered: 2017-09-14
Question
Can you help my question with product M01103-1, anti-Hsp90 alpha Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
B. Taylor
Verified customer
Asked: 2017-07-25
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01103-1 anti-Hsp90 alpha Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-07-25
Question
Is a blocking peptide available for product anti-Hsp90 alpha Rabbit Monoclonal antibody (M01103-1)?
D. Anderson
Verified customer
Asked: 2017-07-04
Answer
We do provide the blocking peptide for product anti-Hsp90 alpha Rabbit Monoclonal antibody (M01103-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-07-04
