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Product Info Summary
| SKU: | M00955-3 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-HSPA5 Antibody
SKU/Catalog Number
M00955-3
Size
50 µl
Description
Boster Bio Anti-HSPA5 Antibody (Catalog # M00955-3). Tested in WB application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-HSPA5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00955-3)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1672CT323.22.82
Isotype
IgG1,k
Immunogen
This HSPA5 antibody is generated from a mouse immunized with a recombinant protein between 420-654 amino acids from human HSPA5.
Reactive Species
M00955-3 is reactive to HSPA5 in Human, Mouse, Rat
Calculated molecular weight
72.3 kDa
Background of HSPA5
Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00955-3 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:20000
Validation Images & Assay Conditions
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All lanes : Anti-HSPA5 Antibody at 1:20000 dilution
Lane 1: HepG2 whole cell lysate
Lane 2: MCF-7 whole cell lysate
Lane 3: Jurkat whole cell lysate
Lane 4: mouse liver lysate
Lane 5: rat liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 72 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-HSPA5 Antibody (M00955-3)
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