Product Info Summary
SKU: | PB9598 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-HSPB2 Antibody Picoband™
SKU/Catalog Number
PB9598
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HSPB2 Antibody Picoband™ catalog # PB9598. Tested in WB applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HSPB2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9598)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human HSPB2 recombinant protein (Position: M1-P182). Human HSPB2 shares 97.3% amino acid (aa) sequence identity with both mouse and rat HSPB2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9598 is reactive to HSPB2 in Human, Rat
Applications
PB9598 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
20 kDa
Calculated molecular weight
20.233kDa
Background of Hspb2
Alpha-B-crystallin is a member of the alpha-crystallin/small heat-shock protein (HSP) family and under various neuropathologic conditions accumulates in reactive astrocytes and degenerating neurons. In the 5-prime flanking region of the CRYAB gene on 11q22-q23, where a constitutive DNaseI hypersensitive site is located, Iwaki et al. (1997) identified a gene transcribed in the opposite direction. Analysis of its mRNA structure by RT-PCR and 5-prime/3-prime RACE revealed that this gene is composed of 2 exons and encodes a new member of the alpha-crystallin/hsp family. They termed the gene HSPB2. Iwaki et al. (1997) also determined the complete genomic structure of the rat homolog of HSPB2. Southern blot analysis demonstrated that HSPB2 is expressed preferentially in skeletal muscle and heart but not in the lens, while the neighboring CRYAB gene is highly expressed in all 3 tissues. The 2 related genes are arranged in a head-to-head manner with an intergenic sequence of less than 1 kb, raising a possibility of shared regulatory elements for their expression.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Rat, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of HSPB2 using anti-HSPB2 antibody (PB9598).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug,
Lane 2: Rat Skeletal Muscle Tissue Lysate at 50ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPB2 antigen affinity purified polyclonal antibody (Catalog # PB9598) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSPB2 at approximately 20 kDa. The expected band size for HSPB2 is at 20 kDa.
Protein Target Info & Infographic
Gene/Protein Information For HSPB2 (Source: Uniprot.org, NCBI)
Gene Name
HSPB2
Full Name
Heat shock protein beta-2
Weight
20.233kDa
Superfamily
small heat shock protein (HSP20) family
Alternative Names
10 kDa chaperonin; 10 kDa heat shock protein, mitochondrial; Chaperonin 10 Homolog; Chaperonin 10; CPN10HSP10; Early-pregnancy factor; EPF; GroES; heat shock 10kD protein 1 (chaperonin 10); heat shock 10kDa protein 1 (chaperonin 10); HSP10; HSPE1 HSPB2 HSP27, Hs.78846, LOH11CR1K, MKBP heat shock protein family B (small) member 2 heat shock protein beta-2|DMPK-binding protein|heat shock 27kDa protein 2|heat-shock protein beta-2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HSPB2, check out the HSPB2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HSPB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HSPB2 Antibody Picoband™ (PB9598)
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No publications found for PB9598
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-HSPB2 Antibody Picoband™
Question
We are currently using anti-HSPB2 antibody PB9598 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on goat tissues as well?
R. Carter
Verified customer
Asked: 2019-07-09
Answer
The anti-HSPB2 antibody (PB9598) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-09