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- Table of Contents
HTATSF1 antibody (A07890-2) performs well in ELISA, Flow Cytometry, IF, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of MCF-7 cells using anti-HTATSF1 antibody (A07890-2).
Overlay histogram showing MCF-7 cells stained with A07890-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HTATSF1 Antibody (A07890-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Overlay histogram showing MCF-7 cells stained with A07890-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HTATSF1 Antibody (A07890-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Anti-HTATSF1 Antibody Picoband®
HTATSF1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IF, IHC, WB |
Tested Applications
HTATSF1 antibody was positively detected by WB in:
human Hela whole cell, human HepG2 whole cell, human Jurkat whole cell, human MCF-7 whole cell, rat brain tissue, rat C6 tissue, mouse brain tissue, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
HTATSF1 antibody was positively detected by IHC in:
mouse brain tissue, mouse brain tissue, mouse brain tissue, mouse brain tissue, mouse cerebellum tissue, mouse cerebellum tissue, rat brain tissue, rat brain tissue, rat brain tissue, rat brain tissue, rat cerebellum tissue, rat cerebellum tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
HTATSF1 antibody was positively detected by IF in:
mouse brain tissue, rat brain tissue
HTATSF1 antibody was positively detected by FC in:
MCF-7 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Mouse, Rat
Immunofluorescence
5 μg/ml
Mouse, Rat
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Recommend
Rat
Tested
Tested
Tested
Recommend
Mouse
Tested
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-HTATSF1 Antibody Picoband®
View all HTATSF1 Antibodies
View all HTATSF1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human HTATSF1 recombinant protein (Position: H126-D333). Human HTATSF1 shares 96.2% amino acid (aa) sequence identity with mouse HTATSF1.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
140 kDa
Calculated Molecular Weight
85.9 kDa
Background of HTATSF1
HIV Tat-specific factor 1 is a protein that in humans is encoded by the HTATSF1 gene. The protein encoded by this gene functions as a cofactor for the stimulation of transcriptional elongation by HIV-1 Tat, which binds to the HIV-1 promoter through Tat-TAR interaction. This protein may also serve as a dual-function factor to couple transcription and splicing and to facilitate their reciprocal activation. Alternatively spliced transcript variants have been found for this gene.
Cite This Product
Anti-HTATSF1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07890-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Rabbit
100%
High Homology
Dog
99%
High Homology
Cow
99%
High Homology
Sheep
98%
High Homology
Mouse
96%
High Homology
Rat
96%
High Homology
Zebrafish
69%
Low homology
Drosophila
52%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for HTATSF1 antibody
Immunohistochemistry (IHC) protocol for HTATSF1 antibody
Immunofluorescence (IF) protocol for HTATSF1 antibody
Flow Cytometry (FC) protocol for HTATSF1 antibody
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Product Promise
We promise Anti-HTATSF1 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
A07890-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026