Anti-Hu CD14 Purified Monoclonal Antibody

Product Name

Anti-Hu CD14 Purified Monoclonal Antibody

View all CD14 Antibodies

SKU/Catalog Number

M00137-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Hu CD14 Purified Monoclonal Antibody (Catalog# M00137-1). Tested in ELISA, IHC-P, Flow Cytometry application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Hu CD14 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00137-1)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

B-A8

Isotype

Mouse IgG1

Immunogen

Human monocytes. The mouse monoclonal antibody B-A8 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked extracellular membrane glycoprotein expressed on the surface of monocytes, macrophages and weakly on granulocytes; also expressed by most tissue macrophages.

Reactive Species

M00137-1 is reactive to CD14 in Human

Observed Molecular Weight

42 kDa

Calculated molecular weight

40.1 kDa

Background of CD14

CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where it serves as a multifunctional lipopolysaccharide receptor. It is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00137-1 is guaranteed for ELISA, Flow Cytometry, IHC-P Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: The antibody B-A8 has been tested as the capture antibody in a sandwich ELISA for analysis of human CD14 in combination with antibody MEM-18 (cat. no. 1B-212-C100). Flow cytometry: 1 μg/ml; Immunohistochemistry (paraffin sections): 2-10 μg/ml, positive tissue: PML brain sections; positive staining on human PML brain sections was mainly observed on monocytes in the lumenal side of brain blood vessels, and on some perivascular cells adjacent to medium-sized vessels. Heat retrieval of antigen is recommeded.

Validation Images & Assay Conditions

See full target information CD14

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