Anti-Hu CD169 Purified SIGLEC1 Monoclonal Antibody

Product Name

Anti-Hu CD169 Purified SIGLEC1 Monoclonal Antibody

View all SIGLEC1 Antibodies

SKU/Catalog Number

M05186

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Hu CD169 Purified SIGLEC1 Monoclonal Antibody (Catalog# M05186). Tested in Flow Cytometry, IP, WB, IHC-F application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Hu CD169 Purified SIGLEC1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05186)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

7-239

Isotype

Mouse IgG1

Immunogen

Human rhinovirus 14-infected monocyte-derived dendritic cells. The mouse monoclonal antibody 7-239 recognizes an extracellular epitope of CD169 (sialoadhesin, Siglec-1), a 210 kDa type I transmembrane glycoprotein expressed on macrophages and dendritic cells.

Cross-reactivity

This antibody does not cross-react with Thy-1.1 alloantigen.

Reactive Species

M05186 is reactive to SIGLEC1 in Human

Observed Molecular Weight

42 kDa

Calculated molecular weight

182.6 kDa

Background of SIGLEC1

CD169, also known as Siglec-1 or sialoadhesin, is a type I transmembrane glycoprotein of the sialic acid binding Ig-like lectin family. It binds to sialylated glycoproteins on various haematopoietic cells to mediate cell-cell interactions. CD169 is expressed on a subset of macrophages and dendritic cells. On CD14+ monocytes its expression can be induced by interferon alpha and gamma. High expression of CD169 is observed in the spleen, lymph nodes, bone marrow, and under inflammatory conditions rheumatoid arthritis and atherosclerosis, lower in the liver, lungs and gut. It has been shown to be involved in antigen presentation to invariant NKT cells, which play an important role in the innate arm of the immune system to modulate the subsequent acquired immune responses.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M05186 is guaranteed for Flow Cytometry, IP, IHC-F, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 1-4 µg/ml

Validation Images & Assay Conditions

See full target information SIGLEC1

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