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Product Info Summary
| SKU: | M00925-2 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, ICC, WB |
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Product info
Product Name
Anti-Hu CD178 Purified FASLG Monoclonal Antibody
SKU/Catalog Number
M00925-2
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD178 Purified FASLG Monoclonal Antibody (Catalog# M00925-2). Tested in Flow Cytometry, IP, WB, ICC application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD178 Purified FASLG Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00925-2)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
NOK-1
Isotype
Mouse IgG1
Immunogen
L5178Y mouse T lymphoma cells expressing recombinant human CD178. The mouse monoclonal antibody NOK-1 recognizes an extracellular epitope of CD178 / Fas-L, an approximately 40 kDa transmembrane glycoprotein expressed on neutrophils, monocytes, and activated T and NK cells.
Reactive Species
M00925-2 is reactive to FASLG in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
31.5 kDa
Background of FASLG
CD178 / Fas-L (Fas ligand, CD95L), a member of TNF family transmembrane glycoproteins, is responsible for induction of apoptosis in cells containing its receptor CD95 / Fas. The CD178-mediated apoptosis pathway has been implicated in peripheral tolerance, tissue pathology, and maintenance of the immune privileged sites. Defects in this interaction may be related to some cases of systemic lupus erythematosus (SLE). CD178 was also described as a co-stimulatory receptor for T-cell activation in mice in vivo.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00925-2 is guaranteed for Flow Cytometry, IP, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 µg/ml
Validation Images & Assay Conditions
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Separation of FasL transfected L5178Y cells stained using anti-human CD178 (NOK-1) purified antibody (concentration in sample 9 µg/ml, GAM APC, red-filled) from FasL transfected L5178Y cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).
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Flow cytometry surface staining pattern of FasL transfected L5178Y cells stained using anti-human CD178 (NOK-1) purified antibody (concentration in sample 9 µg/ml) GAM APC.
Specific Publications For Anti-Hu CD178 Purified FASLG Monoclonal Antibody (M00925-2)
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