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- Table of Contents
CCR5 antibody (A00061-2) performs well in ELISA, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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Western blot analysis of CCR5 using anti-CCR5 antibody (A00061-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: mouse spleen tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCR5 antigen affinity purified polyclonal antibody (Catalog # A00061-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCR5 at approximately 40KD. The expected band size for CCR5 is at 40KD.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: mouse spleen tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCR5 antigen affinity purified polyclonal antibody (Catalog # A00061-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCR5 at approximately 40KD. The expected band size for CCR5 is at 40KD.
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Anti-CCR5 Picoband® Antibody
CCR5 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, WB |
Tested Applications
CCR5 antibody was positively detected by WB in:
rat spleen tissue, mouse spleen tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Mouse, Rat
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
ELISA
Rat
Tested
Recommend
Mouse
Tested
Recommend
Human
Recommend
Monkey
Predicted
Predicted
Product Information
Product Name
Anti-CCR5 Picoband® Antibody
View all CCR5 Antibodies
View all CCR5 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CCR5 recombinant protein (Position: Q21-K322).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
40 kDa
Calculated Molecular Weight
40.5 kDa
Background of CCR5
C-C chemokine receptor type 5, also known as CCR5 or CD195, is a protein on the surface of white blood cells that is involved in the immune system as it acts as a receptor for chemokines. It is mapped to 3p21.31. This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. This protein is expressed by T cells and macrophages, and is known to be an important co-receptor for macrophage-tropic virus, including HIV, to enter host cells. Defective alleles of this gene have been associated with the HIV infection resistance. The ligands of this receptor include monocyte chemoattractant protein 2 (MCP-2), macrophage inflammatory protein 1 alpha (MIP-1 alpha), macrophage inflammatory protein 1 beta (MIP-1 beta) and regulated on activation normal T expressed and secreted protein (RANTES). Expression of this gene was also detected in a promyeloblastic cell line, suggesting that this protein may play a role in granulocyte lineage proliferation and differentiation. This gene is located at the chemokine receptor gene cluster region. An allelic polymorphism in this gene results in both functional and non-functional alleles; the reference genome represents the functional allele. Two transcript variants encoding the same protein have been found for this gene.
Cite This Product
Anti-CCR5 Picoband® Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00061-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Cow
87%
Moderate Homology
Sheep
86%
Moderate Homology
Mouse
84%
Moderate Homology
Rat
84%
Moderate Homology
Dog
84%
Moderate Homology
Rabbit
82%
Moderate Homology
Zebrafish
44%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CCR5 antibody
Publications
Application:
Species:
Chemokine CCL8 and its receptor CCR5 in the spinal cord are involved in visceral pain induced by experimental colitis in mice
Published: 2017-10-14
Journal: BRAIN RESEARCH BULLETIN
Application: IHC,WB
Impact Factor: 3.03
Species: Mouse
PMID: 29037608
The Significance for Chronic Rejection of Cardiac Allograft of Regulated Upon Activation Normal T-cell Cytokine and Its CCR5 Receptor
Published: 2013-03-15
Journal: TRANSPLANTATION PROCEEDINGS
Application: IHC
Impact Factor: 0.95
Species: Rat
PMID: 23498802
Blocking the CC Chemokine Receptor 5 Pathway by Antisense Peptide Nucleic Acid Prolongs Islet Allograft Survival
Published: 2007-01-31
Journal: TRANSPLANTATION PROCEEDINGS
Application: WB
Impact Factor: 0.96
Species: Mouse
PMID: 17275502
Review
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1 Review For Anti-CCR5 Picoband® Antibody
Anti-CCR5 Antibody (A00061-2) produced a clear and specific band at the expected molecular weight with minimal background, and showed higher CCR5 expression in the lung metastatic tumor tissue than in normal lung tissue by Western blot.
| SKU | A00061-2 |
|---|---|
| Application | Western Blot |
| Sample | Normal lungs and lung cancer metastases in nude mice |
| Sample Processing Description | ① Normal lungs of nude mice ② Lung tissue was harvested after nude mice underwent lung cancer xenografts following A549 cell injection in the tail. Total protein was extracted. |
| Other Reagents | RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer |
| Primary Antibody | CCR5 Picoband® Antibody |
| Primary Incubation | 1:2000, overnight at 4 ℃ |
| Secondary Antibody | HRP-conjugated goat anti-rabbit IgG |
| Secondary Incubation | 1:10000, 1 hour in RT |
| Detection | Substrate: ECL substrate; Image system: ChemiDoc MP |
| Results Summary | CR5 protein, short for C-C chemokine receptor type 5, is a G protein-coupled receptor (GPCR) located on the surface of immune cells. It plays a role in regulating the migration, proliferation, and immunity of T cells and monocyte/macrophage cell lines. The results of this experiment showed that the level of CCR5 in metastatic tumor samples was significantly higher than that in normal tissues. |
Yanlin Zhang, Shanxi Medical University
Verified customer
Submitted 2026-07-08
Product Promise
We promise Anti-CCR5 Picoband® Antibody performs excellently in relevant applications for ELISA, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00061-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 12, 2026